This material is not reported as being light sensitive.
SML1046
CHIR99021
≥98% (HPLC), powder, GSK-3 inhibitor
Synonym(s):
6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile, CT99021
Sign Into View Organizational & Contract Pricing
About This Item
Empirical Formula (Hill Notation):
C22H18Cl2N8
CAS Number:
Molecular Weight:
465.34
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.77
Skip To
Product Name
CHIR99021, ≥98% (HPLC)
Quality Level
assay
≥98% (HPLC)
form
powder
color
white to light brown
solubility
DMSO: 2 mg/mL, clear (warmed)
storage temp.
−20°C
SMILES string
ClC(C=C1Cl)=CC=C1C2=NC(NCCNC(N=C3)=CC=C3C#N)=NC=C2C4=NC(C)=CN4
InChI
1S/C22H18Cl2N8/c1-13-10-29-21(31-13)17-12-30-22(32-20(17)16-4-3-15(23)8-18(16)24)27-7-6-26-19-5-2-14(9-25)11-28-19/h2-5,8,10-12H,6-7H2,1H3,(H,26,28)(H,29,31)(H,27,30,32)
InChI key
AQGNHMOJWBZFQQ-UHFFFAOYSA-N
Related Categories
1 of 4
This Item | SML1094 | C9873 | 361559 |
|---|---|---|---|
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥97% (HPLC) |
| form powder | form powder | form powder | form solid |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility DMSO: 2 mg/mL, clear (warmed) | solubility DMSO: 0.5 mg/mL, clear (warmed) | solubility DMSO: >10 mg/mL | solubility DMSO: 5 mg/mL |
| color white to light brown | color white to brown | color white to beige | color light beige |
General description
CHIR99021 is an aminopyrimidine derivative.[1] CHIR99021 promotes self-renewal potential of embryonic stem cells (ESCs) of mice by inhibiting glycogen synthase kinase-3 (GSK-3) activity and potentiates the upregulation of β-catenin and c-Myc functions.[2] CHIR99021 promotes self-renewal of ESCs by modulating transforming growth factor β (TGF-β), Notch and mitogen-activated protein kinases (MAPK) signaling pathways.[1] CHIR99021 is an agonist of wingless/integrated (wnt) signalling, upregulates cyclinA expression and promotes cell proliferation in non-small-cell lung cancer (NSCLC) cell lines.[3]
Application
CHIR99021 has been used as a Wnt signaling agonist to study its effects on the differentiation of human pluripotent stem cell-derived cardiomyocytes (hPSCs).[4][5]
CHIR99021 has been used:
Biochem/physiol Actions
CHIR99021 is a very potent inhibitor of Glycogen synthase kinase 3 (GSK3, IC50 values for GSK3α and GSK3β = 10 and 6.7 nM, respectively). There is no cross-reactivity against CDKs. CHIR99201 potentiates self-renewal of human and mouse embryonic stem cells, and enhances reprogramming of somatic cells into stem cells.
CHIR99021 is a very potent inhibitor of Glycogen synthase kinase 3.
related product
Product No.
Description
Pricing
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
No data available
flash_point_c
No data available
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The roles of Notch3 on the cell proliferation and apoptosis induced by CHIR99021 in NSCLC cell lines: a functional link between Wnt and Notch signaling pathways
Li C, et al.
PLoS ONE, 8(12), e84659-e84659 (2013)
CHIR99021 promotes self-renewal of mouse embryonic stem cells by modulation of protein-encoding gene and long intergenic non-coding RNA expression
Wu Y, et al.
Experimental Cell Research, 319(17), 2684-2699 (2013)
Co-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells
Shy BR
Nucleic Acids Research, 44, 7997-8010 (2016)
Enterochromaffin Cells Are Gut Chemosensors that
Couple to Sensory Neural Pathways
Couple to Sensory Neural Pathways
Bellono NW
Cell, 170, 185-198 (2017)
Chemical conversion of human lung fibroblasts into neuronal cells
Wan XY, et al.
International Journal of Molecular Medicine, 41(3), 1463-1468 (2018)
Articles
Naive pluripotent stem cells cultured in vitro using specialized media and inhibitors mimic "ground-state" cells from blastocysts.
Human pancreatic cancer organoid biobank (PDAC organoids) with various KRAS mutations to aide in 3D cell culture and cancer research applications.
Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
Protocols
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
-
Is CHIR99021 light sensitive?
1 answer-
Helpful?
-
-
What is the solubility of Product SML1046?
1 answer-
The solubility of Product SML1046 has been tested in DMSO at 2 mg/ml when warmed. Although testing in water has not been conducted, it is expected to be less than 1 mg/ml at 25°C, similar to its solubility in saline. Additionally, solubility testing in ethanol has also shown a solubility of less than 1 mg/ml at 25°C.
Helpful?
-
-
How is Product SML1046, CHIR99021, to be dissolved? And once dissolved, is the solution stable?
1 answer-
This product is soluble in DMSO (warmed) at 2-5 mg/mL. Solubility in polar solvents such as water, saline, or ethanol, is expected to be <1 mg/ml at 25° C. Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 6 months at -20°C. Avoid repeated freeze/thaw cycles and protect from light.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service