SML1627

DFHBI

• Synonym(s): (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl- 4H-Imidazol-4-one, (Z)-4-(3,5-Difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one
• Empirical Formula (Hill Notation): C₁₂H₁₀F₂N₂O₂
• CAS Number: 1241390-29-3
• Molecular Weight: 252.22
• PubChem Substance ID: 329825511
• NACRES: NA.77

Properties

• Assay: ≥98% (HPLC)
• Quality Level: 100
• form: powder
• color: yellow to orange
• solubility: DMSO: 20 mg/mL, clear
• storage temp.: −20°C
• SMILES string: FC1=CC(/C=C2N=C(C)N(C)C\2=O)=CC(F)=C1O
• InChI: 1S/C12H10F2N2O2/c1-6-15-10(12(18)16(6)2)5-7-3-8(13)11(17)9(14)4-7/h3-5,17H,1-2H3/b10-5-
• InChI key: ZDDIJYXDUBFLID-YHYXMXQVSA-N

Description

Biochem/physiol Actions

3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) is non fluorescent when unbound.

DFHBI is a membrane permeable and nontoxic fluorogen, which produces fluorescence upon binding. RNA aptamers Spinach, Spinach2 and Broccoli. DFHBI displays fluorescence excitation maxima of 447 nm and peak fluorescence emission of 501 nm. DFHBI is used for imaging RNA in cells.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315 - H319 - H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable