SML1735

PFM01

Name: PFM01
Brand: SIGMA
Price: 131 USD
Availability: InStock

≥98% (HPLC)

Synonym(s):

(5Z)-5-[(4-Hydroxyphenyl)methylene]-3-(2-methylpropyl)-2-thioxo-4-thiazolidinone, 5-(4-Hydroxybenzylidene)-3-isobutyl-2-thioxothiazolidin-4-one

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₅NO₂S₂

CAS Number:

1558598-41-6

Molecular Weight:

293.40

MDL number:

MFCD02220972

UNSPSC Code:

12352200

PubChem Substance ID:

329826045

NACRES:

NA.77

Key Documents

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Quality Level

100

assay

≥98% (HPLC)

form

powder

color

white to beige

solubility

DMSO: 20 mg/mL, clear

storage temp.

2-8°C

SMILES string

OC1=CC=C(/C=C2SC(N(CC(C)C)C\2=O)=S)C=C1

InChI

1S/C14H15NO2S2/c1-9(2)8-15-13(17)12(19-14(15)18)7-10-3-5-11(16)6-4-10/h3-7,9,16H,8H2,1-2H3/b12-7-

InChI key

GPURHDUTZUYAFI-GHXNOFRVSA-N

Related Categories

Bioactive Small Molecule Inhibitors

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Show Differences

1 of 4

This Item	SML1839	SML2688	SML1906
Sigma-Aldrich SML1735 PFM01	Sigma-Aldrich SML1839 PFM39	Sigma-Aldrich SML2688 J30-8	Sigma-Aldrich SML1906 AS1517499
assay ≥98% (HPLC)	assay ≥98% (HPLC)	assay ≥98% (HPLC)	assay ≥98% (HPLC)
form powder	form powder	form powder	form powder
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
solubility DMSO: 20 mg/mL, clear	solubility DMSO: 15 mg/mL, clear	solubility DMSO: 2 mg/mL, clear	solubility DMSO: 20 mg/mL, clear
color white to beige	color yellow to orange	color white to beige	color white to beige

Biochem/physiol Actions

PFM01 is a cell-permeable N-alkylated Mirin (Sigma Cat. No. 475954) derivative that selectively inhibits against MRE11 endo-, but not exo-, nuclease activity. PFM01 targets MRE11 at a site near the dimer interface, distinct from that occupied by Mirin and PFM39 to allow disruption of the ssDNA-binding groove and selective inhibition against MRE11 endo-, but not exo-, nuclease activity. While both endonuclease and exonuclease activities are required for MRE11-mediated homologous recombination (HR) repair, only FM01 (100 μM), but not the exonuclease inhibitors Mirin (500 μM) and PFM39 (100 μM), rescues G2-phase double-strand break (DSB) repair defect in HR protein BRCA2-deficient HSC62-hTERT fibroblasts following ionizing irradiation (IR) by blocking HR initiation and thereby allowing non-homologous end joining (NHEJ) to proceed.

PFM01 is a cell-permeable N-alkylated Mirin derivative that selectively inhibits against MRE11 endo-, but not exo-, nuclease activity.

pictograms

GHS07

signalword

Warning

hcodes

H302,H315,H319

pcodes

P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Small-Molecule Inhibitors Targeting DNA Repair and DNA Repair Deficiency in Research and Cancer Therapy.

Sarah R Hengel et al.

Cell chemical biology, 24(9), 1101-1119 (2017-09-25)

To maintain stable genomes and to avoid cancer and aging, cells need to repair a multitude of deleterious DNA lesions, which arise constantly in every cell. Processes that support genome integrity in normal cells, however, allow cancer cells to develop

RAD50 regulates mitotic progression independent of DNA repair functions.

Lea Völkening et al.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(2), 2812-2820 (2020-01-08)

The Mre11A/RAD50/NBN complex (MRN) is an essential regulator of the cellular damage response after DNA double-strand breaks (DSBs). More recent work has indicated that MRN may also impact on the duration of mitosis. We show here that RAD50-deficient fibroblasts exhibit

Super-resolution mapping of cellular double-strand break resection complexes during homologous recombination.

Donna R Whelan et al.

Proceedings of the National Academy of Sciences of the United States of America, 118(11) (2021-03-13)

Homologous recombination (HR) is a major pathway for repair of DNA double-strand breaks (DSBs). The initial step that drives the HR process is resection of DNA at the DSB, during which a multitude of nucleases, mediators, and signaling proteins accumulates

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PFM01