SML2697

DFHBI-1T

≥98% (HPLC)

• Synonym(s): (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one, (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4H)-one, DFHBI 1T
• Empirical Formula (Hill Notation): C₁₃H₉F₅N₂O₂
• CAS Number: 1539318-36-9
• Molecular Weight: 320.21
• MDL number: MFCD31628972

Properties

• Assay: ≥98% (HPLC)
• form: powder
• storage condition: protect from light
• color: white to beige
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

DFHBI-IT is a membrane-permeable, non-cytotoxic, RNA aptamers-activated fluorescence probe widely employed in varous detection systems utilizing compatible RNA tags such as Spinach, Spinach2, iSpinach, and Broccoli. Upon binding Spinach2, DFHBI-1T offers brighter fluorescence than DFHBI due to increased extinction coefficient & quantum yield (by 1.4- and 1.3-fold, respectively). DFHBI-1T is also better suited for devices optimized for GFP or fluorescein detection/imaging due to its red shift in excitation & emission wavelengths (Spinach2-comlex Ex/Em = 482/505 nm with DFHBI-1T vs 447/501 nm with DFHBI).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable