Too much residual medium or cell mass may lead to recovery of some large RNAs. It is critical to remove as much residual medium as possible by re-centrifuging the pellet. One can also test different ratios of the lysis mix (Small RNA Lysis Buffer and Binding Solution).
SNC10
mirPremier® microRNA Isolation Kit
1 sufficient for 10 preparations
Synonym(s):
microRNA Isolation Kit
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UNSPSC Code:
41105501
NACRES:
NA.52
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$95.92
List Price$110.00Save 13%Web-Only Promotion
In StockDetails
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This Item | RTN350 | RTN10 | RTN70 |
|---|---|---|---|
| usage sufficient for 10 preparations | usage sufficient for 350 purifications | usage sufficient for 10 purifications | usage sufficient for 70 purifications |
| technique(s) DNA extraction: suitable | technique(s) RNA purification: suitable | technique(s) - | technique(s) RNA purification: suitable |
SNC50 | RTN10, RTN70, RTN9602, RTN9604 | RTN350, RTN70, RTN9602, RTN9604 | RTN10, RTN350, RTN9602, RTN9604 |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Application
Features and Benefits
- Developed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological samples.
- Facilitates rapid and effective extraction and concentration of miRNA in 30 minutes for downstream applications.
- Capable of extracting miRNA with high purity with no detectable large RNA.
- Hazardous organic extractions are not involved.
Legal Information
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
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Warning
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
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How can I avoid co-purifying mRNAs and rRNAs when purifying small RNAs from gram negative bacteria using mirPremier® microRNA Isolation Kit?
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Can I purify 18S and 28S large RNAs along with miRNAs with the mirPremier® microRNA Isolation Kit?
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Large RNAs (18S and 28S) from the pellet fraction can be purified after transferring the microRNA-containing supernatant to a new tube by using the total RNA protocol or by phenol/chloroform extraction.
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Can the mirPremier® microRNA Isolation Kit be used to isolate small RNAs from purified total RNA?
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We have used to the kit to purify in vitro transcribed small RNAs with great success, but have not done so with purified total RNA. Here are the steps we would recommend trying with purified total RNA:1. Prepare a lysis mix with 0.7 vol. Small RNA Lysis Buffer (M1070) and 0.3 vol. Binding Solution (L8042).2. Add 500 ul of lysis mix with 50 ul total RNA and mix thoroughly.3. Spin at 14000 rpm for 5 min to precipitate large RNA.5. Transfer the supernatant to a new tube.6. Add 610 ul (1.1 vol.) 100% ethanol to the supernatant and mix well.7.Transfer the mixture to a binding column and spin 1 min to bind. Repeat the binding step with the remaining mixture.8. Wash the column first with 700 ul 100% ethanol, and then with ethanol-diluted wash Solution 2.9. Dry the column and elute small RNA.
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Has the mirPremier® microRNA Isolation Kit been tested on sperm cells?
1 answer-
We have not tested mirPremier™ microRNA Isolation Kit with sperm cells.
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