Skip to Content
MilliporeSigma

SRE0066

Sigma-Aldrich

Guanidine hydrochloride solution, 6M

Synonym(s):

Aminoformamidine hydrochloride, GuHCl, Guanidinium chloride, Guanidium chloride

Slide 1 of 1
Sign Into View Organizational & Contract Pricing

Select a Size

100 ML
$262.00
500 ML
$830.00
4 L
$1,620.00

$262.00

Available to ship TODAYDetails

Request a Bulk Order
Ships Every 4 weeks

About This Item

UNSPSC Code:
12352209
NACRES:
NA.25

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

liquid

Quality Level

500

concentration

6 M

impurities

<100 cfu/mL Bioburden

color

colorless

pH

4.5—5.5

density

1.1 —1.3  g/cm3

application(s)

diagnostic assay manufacturing

storage temp.

room temp

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
G72945093750950
Sigma-Aldrich

Sigma-Aldrich

SRE0066

Guanidine hydrochloride solution, 6M

Guanidine hydrochloride solution 8&#160;M, pH 8.5, buffered aqueous solution

Sigma-Aldrich

G7294

Guanidine hydrochloride solution

Guanidine hydrochloride solution BioUltra, ~8&#160;M in H2O

Sigma-Aldrich

50937

Guanidine hydrochloride solution

Guanidine hydrochloride &#8805;98%

Sigma-Aldrich

50950

Guanidine hydrochloride

concentration

6 M

concentration

8 M

concentration

~8 M in H2O

concentration

-

Quality Level

500

Quality Level

200

Quality Level

100

Quality Level

200

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

form

liquid

form

buffered aqueous solution

form

liquid

form

powder or crystals

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

Application

Guanidine hydrochloride is commonly used as a denaturant, because of its ability to break hydrogen bonds between amino acid residues. By breaking these bonds, the 3D conformation of the protein is unfolded and the aqueous solubility of the protein is greatly increased. Once denatured, the protein can be easily reduced, modified, or analyzed, in a variety of procedures. Further processing of the sample can allow the proteins to be analyzed by other common protein analysis methods including mass spectrometry, electrophoresis, and enzymatic digests.
Guanidine hydrochloride has also typically been used for the isolation of RNA, to denature globular proteins, and for protein refolding studies. It can also be used to facilitate the generation of tryptic peptides for analysis of complex protein samples.

Physical form

This product is a ready-to-use 6 M guanidine hydrochloride solution. The 6 M Guanidine hydrochloride solution may be used as a stock solution to prepare solutions of lower concentrations as appropriate.

Note: If precipitation is present in the 6 M buffer, warm the bottle to 37 °C and mix until completely dissolved prior to use.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000439783
0000439783
0000444673
0000444673
0000458838

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jun-Yi Zhu et al.
Autophagy, 17(10), 3160-3174 (2021-01-07)
We investigated in larval and adult Drosophila models whether loss of the mitochondrial chaperone Hsc70-5 is sufficient to cause pathological alterations commonly observed in Parkinson disease. At affected larval neuromuscular junctions, no effects on terminal size, bouton size or number
Georgios I Laliotis et al.
Nature communications, 12(1), 4624-4624 (2021-08-01)
AKT-phosphorylated IWS1 regulates alternative RNA splicing via a pathway that is active in lung cancer. RNA-seq studies in lung adenocarcinoma cells lacking phosphorylated IWS1, identified a exon 2-deficient U2AF2 splice variant. Here, we show that exon 2 inclusion in the
Kaamini M Dhanabalan et al.
Biomaterials science, 8(15), 4308-4321 (2020-07-01)
Osteoarthritis (OA) is a joint disease characterized by progressive damage of articular cartilage and the adjoining subchondral bone. Chondrocytes, the primary cells of the cartilage, have limited regenerative capacity and when they undergo stress due to trauma or with aging
Jingwen Yang et al.
Science signaling, 14(665) (2021-01-14)
Cranial neural crest cells (CNCCs) are a population of multipotent stem cells that give rise to craniofacial bone and cartilage during development. Bone morphogenetic protein (BMP) signaling and autophagy have been individually implicated in stem cell homeostasis. Mutations that cause
Yoshinori Aso et al.
eLife, 8 (2019-11-15)
Animals employ diverse learning rules and synaptic plasticity dynamics to record temporal and statistical information about the world. However, the molecular mechanisms underlying this diversity are poorly understood. The anatomically defined compartments of the insect mushroom body function as parallel

Articles

Workflow for the Analysis of Polysorbate 80 in Erbitux® Formulation

A complete reversed phase HPLC-ELSD workflow, including sample preparation for the analysis of Polysorbate 80 in a monoclonal antibody formulation.

Biosimilar Comparability Workflow for Intact Mass Analysis

A RPLC-MS workflow for mass analysis of non-reduced monoclonal antibody, trastuzumab, including calibration, and system suitability tests.

Middle Up Mass Analysis Using Trastuzumab as Model Protein

A RPLC-MS workflow for mass analysis of reduced monoclonal antibody, trastuzumab, including reduction procedures, calibration, and system suitability tests.

Protocols

Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody

A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.

High-Throughput Glycoprofiling Workflow for Rituximab

SEC-MS protocol for rapid glycoprofiling of monoclonal antibodies with antibody purification and mass spectrometer calibration.

Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is the aqueous solution of guanidine HCl considered a buffer, or is it prepared as an aqueous solution and not in a buffer?

    1 answer

    1. Guanidine is a strong organic base, making SRE0066 an aqueous solution. In technical terms, guanidine has a pKa of 13.6, indicating that it is a strong basic buffer.

      Helpful?

  4. What is the container plastic type for SRE0066-4L?

    1 answer

    1. The item is packaged in a polyethylene terephthalate glycol container.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service