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SRE0068

Sigma-Aldrich

SSC Buffer, 20X Concentrate

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Synonym(s):
Saline Sodium Citrate Buffer

form

liquid

Quality Level

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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This Item
S6639S2015T6789
Sigma-Aldrich

SRE0068

SSC Buffer, 20X Concentrate

SSC Buffer 20× Concentrate for Northern and Southern blotting, solution

S6639

SSC Buffer 20× Concentrate

Sigma-Aldrich

S2015

SSPE Buffer 20× Concentrate

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

form

liquid

form

solution

form

solution

form

powder

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

storage temp.

2-8°C

Application

SSC Buffer, 20X Concentrate, is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5X. It is also for use in preparing nucleic acids for hybridization.

Physical form

This SSC Buffer, 20X Concentrate, contains 0.3 M sodium citrate in 3 M NaCl. Preparation of 1X SSC working buffer: Dilute the 20X concentrated buffer 20-fold with ultrapure water (= 18 MΩ x cm resistivity at 25 °C).

Notes: If precipitation is present in the 20X concentrated buffer, warm the bottle to 37 °C and mix until completely dissolved prior to dilution. It is recommended 1X working solutions be filtered through a 0.2 mm filter before use.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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