SRE0111

Heat-labile Cod Uracil-DNA Glycosylase

Name: Heat-labile Cod Uracil-DNA Glycosylase
Brand: SIGMA

recombinant, expressed in E. coli, Triton-free

Synonym(s):

UDG, UNG

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About This Item

NACRES:

NA.54

UNSPSC Code:

41106300

Key Documents

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Product Name

Heat-labile Cod Uracil-DNA Glycosylase, recombinant, expressed in E. coli, Triton-free

recombinant

expressed in E. coli

composition

Triton-free

packaging

pkg of 100 U
pkg of 1000 U

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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This Item	11444646001	UDGHL-RO	71086
Sigma-Aldrich SRE0111 Heat-labile Cod Uracil-DNA Glycosylase	Roche 11444646001 Uracil-DNA Glycosylase	Roche UDGHL-RO Uracil-DNA Glycosylase, heat-labile	Sigma-Aldrich 71086 KOD Hot Start DNA Polymerase
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in dry ice	shipped in -	shipped in -	shipped in dry ice
packaging pkg of 100 U, pkg of 1000 U	packaging pkg of 100 U	packaging pkg of 100 U (11775367001), pkg of 500 U (11775375001)	packaging -
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100, 200
composition Triton-free	composition -	composition -	composition -

Application

Heat-labile Cod Uracil-DNA Glycosylase eliminates carry-over contamination in PCR, RT-PCR, RT-qPCR, qPCR and RT-LAMP

Features and Benefits

Heat inactivation: The only UNG that is completely and irreversibly heat inactivated thus ensuring long-term sample integrity for subsequent post-PCR analysis such as cloning, sequencing or genotyping
Cod UNG is not active on uracil in RNA.
High sensitivity: Remove more than 108 copies of contaminating DNA without affecting the sensitivity (Cq) of the assay
Simple protocol: Applying Cod UNG in a RT-PCR is done by a 5 min Cod UNG preincubation step at RT prior to the amplification reaction

General description

Uracil-DNA glycosylases are DNA repair enzymes that participate in the base excision repair pathway.[1] Uracil DNA Glycosylase, also known as UNGor UDG, is an enzyme that hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic(apyrimidinic) site in DNA. Contamination of PCR samples with amplicon DNA generated by previous reactions, “carry-over contamination”, is one of the major sources of false positive results. Heat-labile Cod Uracil-DNA Glycosylase prevents carry-over contamination in PCR, qPCR, RT-qPCR, and RT-LAMP assays. The enzyme is produced in a recombinant E. coli (ung–) strain that contains a modified Cod UNGgene.

Other Notes

One Unit is defined as the amount of enzyme that liberates 1 nmol Uracil per hour from Uracil-labelled DNA at 37 °C in 70 mm Tris-HCI pH 8.0 (@25 °C), 10 mM NaCI, 1 mM EDTA and 0.1 mg/ml BSA.

This product is for R&D use only. Not for drug, household, or other uses.

Preparation Note

In solution

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000509466

0000509432

0000509558

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Uracil-DNA glycosylases?Structural and functional perspectives on an essential family of DNA repair enzymes

N Schormann et al.

Protein Science, 1667?1685-1667?1685 (2014)

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