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SRE0111

Sigma-Aldrich

Heat-labile Cod Uracil-DNA Glycosylase

recombinant, expressed in E. coli, Triton-free

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Synonym(s):
UDG, UNG

recombinant

expressed in E. coli

Quality Level

200

composition

Triton-free

packaging

pkg of 100 U
pkg of 1000 U

shipped in

dry ice

storage temp.

−20°C

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This Item
SRP6125SRP6112SRP6045
Heat-labile Cod Uracil-DNA Glycosylase recombinant, expressed in E. coli, Triton-free

Sigma-Aldrich

SRE0111

Heat-labile Cod Uracil-DNA Glycosylase

Glyoxalase I human recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

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SRP6125

Glyoxalase I human

G6PD from E. coli recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

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SRP6112

G6PD from E. coli

Sigma-Aldrich

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SRP6045

Glucokinase from human liver

packaging

pkg of 100 U, pkg of 1000 U

packaging

pkg of 100 μg

packaging

pkg of 100 μg

packaging

pkg of 10 μg

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

composition

Triton-free

composition

-

composition

-

composition

-

General description

Uracil-DNA glycosylases are DNA repair enzymes that participate in the base excision repair pathway. Uracil DNA Glycosylase, also known as UNGor UDG, is an enzyme that hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic(apyrimidinic) site in DNA. Contamination of PCR samples with amplicon DNA generated by previous reactions, “carry-over contamination”, is one of the major sources of false positive results. Heat-labile Cod Uracil-DNA Glycosylase prevents carry-over contamination in PCR, qPCR, RT-qPCR, and RT-LAMP assays. The enzyme is produced in a recombinant E. coli (ung–) strain that contains a modified Cod UNGgene.

Application

Heat-labile Cod Uracil-DNA Glycosylase eliminates carry-over contamination in PCR, RT-PCR, RT-qPCR, qPCR and RT-LAMP

Features and Benefits

  • Heat inactivation: The only UNG that is completely and irreversibly heat inactivated thus ensuring long-term sample integrity for subsequent post-PCR analysis such as cloning, sequencing or genotyping
  • Cod UNG is not active on uracil in RNA.
  • High sensitivity: Remove more than 108 copies of contaminating DNA without affecting the sensitivity (Cq) of the assay
  • Simple protocol: Applying Cod UNG in a RT-PCR is done by a 5 min Cod UNG preincubation step at RT prior to the amplification reaction

Unit Definition

One Unit is defined as the amount of enzyme that liberates 1 nmol Uracil per hour from Uracil-labelled DNA at 37 °C in 70 mm Tris-HCI pH 8.0 (@25 °C), 10 mM NaCI, 1 mM EDTA and 0.1 mg/ml BSA.

Reconstitution

In solution

Other Notes

This product is for R&D use only. Not for drug, household, or other uses.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Uracil-DNA glycosylases?Structural and functional perspectives on an essential family of DNA repair enzymes
N Schormann et al.
Protein Science, 1667?1685-1667?1685 (2014)

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