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SRE0112

Sigma-Aldrich

Heat-labile Cod Uracil-DNA Glycosylase

recombinant, expressed in E. coli, Triton-free and Glycerol-free

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Synonym(s):
UDG, UNG

recombinant

expressed in E. coli

Quality Level

200

composition

Triton-free and Glycerol-free

shipped in

dry ice

storage temp.

−20°C

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This Item
UDGECU9257D9310
Heat-labile Cod Uracil-DNA Glycosylase recombinant, expressed in E. coli, Triton-free and Glycerol-free

Sigma-Aldrich

SRE0112

Heat-labile Cod Uracil-DNA Glycosylase

Sigma-Aldrich

Sigma-Aldrich

UDGEC

E. coli Uracil-DNA Glycosylase (UDG)

Sigma-Aldrich

Sigma-Aldrich

U9257

UbcH9 Conjugating Enzyme human

Sigma-Aldrich

Sigma-Aldrich

D9310

Glycerol-free JumpStart™ Taq DNA Polymerase

composition

Triton-free and Glycerol-free

composition

-

composition

-

composition

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

General description

Uracil-DNA glycosylases are DNA repair enzymes that participate in the base excision repair pathway. UracilDNA Glycosylase, also known as UNG or UDG, is an enzyme that hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an abasic (apyrimidinic) site in DNA. Contamination of PCR samples with amplicon DNA generated by previous reactions, “carry-over contamination”, is one of the major sources of false positive results. Heat-labile Cod Uracil-DNA Glycosylase prevents carry-over contamination in PCR, qPCR, RT-qPCR, and RT-LAMP assays. The enzyme is produced in a recombinant E.coli (ung–) strain that contains a modified Cod UNG gene.

Specificity

One Unit is defined as the amount of enzyme that liberates 1 nmol Uracil per hour from Uracil-labelled DNA at 37°C in 70 mm Tris-HCI pH 8.0 (@25°C), 10 mM NaCI, 1 mM EDTA and 0.1 mg/ml BSA.

Application

Heat-labile Cod Uracil-DNA Glycosylase eliminates carry-over contamination in PCR, RT-PCR, RT-qPCR, qPCR and RT-LAMP

Features and Benefits

  • Heat inactivation: The only UNG that is completely and irreversibly heat inactivated thus ensuring long-term sample integrity for subsequent post-PCR analysis such as cloning, sequencing or genotyping
  • Cod UNG is not active on uracil in RNA.
  • High sensitivity: Remove more than 108 copies of contaminating DNA without affecting the sensitivity (Cq) of the assay
  • Simple protocol: Applying Cod UNG in a RT-PCR is done by a 5 min Cod UNG preincubation step at RT prior to the amplification reaction

Reconstitution

In solution

Other Notes

This product is for R&D use only. Not for drug, household, or other uses.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Uracil-DNA glycosylases?structural and functional perspectives on an essential family of DNA repair enzymes
Schormann N, et al.
Protein Science, 23(12), 1667-1685 (2014)

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