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SRP0120

Sigma-Aldrich

Sirtuin 6 human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym(s):

NAD-dependent ADP-ribosyltransferase sirtuin-6, SIR2L6, SIRT6, sir2-like 6

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

65 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SIRT6(51548)

General description

Sirtuin 6 (Sir6) is encoded by the gene mapped to human chromosome 19p13.3, a region linked with development of acute leukemia. Sir 6 is mainly expressed in bone cells and in the ovaries, but it is absent in the bone marrow. Sirt6 is a chromatin-associated protein localized specifically in nucleus.
Human Sirtuin 6, GenBank Accession No. NM_016539), full length with N-terminal ST tag, MW = 65kDa, expressed in Escherichia coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Sirtuin 6 (Sir6) is a mono-ADP-ribosyltransferase, which catalyzes the transfer of ADP-ribose moiety from NAD to itself and histones. It has been implicated in the regulation of DNA repair, genomic stability and telomere integrity, gene expression, and metabolism. In addition, it also prevents age-related disorders and premature aging. Sir6 plays a vital role in the inhibition of nuclear factor-κB (NF-κB)–mediated inflammatory responses in rheumatoid joints. Hence, Sir6 is considered to be a potent target for treatment of rheumatoid arthritis (RA). Sir6 is also majorly implicated in the regulation of glucose and fat metabolism. Sir6 prevent cardiac hypertrophy in vitro via inhibiting NF-κB-dependent transcriptional activity and this effect is relied on its deacetylase activity.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chromosomal organization and fluorescence in situ hybridization of the human Sirtuin 6 gene.
Mahlknecht U
International Journal of Oncology, 28(2), 447-456 (2006)
Neural sirtuin 6 (Sirt6) ablation attenuates somatic growth and causes obesity.
Schwer B
Proceedings of the National Academy of Sciences of the USA, 107(50), 21790-21794 (2010)
Abhishek Bhardwaj et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(5), E538-E547 (2016-01-21)
SIRT6 (sirtuin 6) is a member of sirtuin family of deacetylases involved in diverse processes including genome stability, metabolic homeostasis, and tumorigenesis. However, the role of SIRT6 deacetylase activity in its tumor-suppressor functions is not well understood. Here we report
Overexpression of sirtuin 6 suppresses inflammatory responses and bone destruction in mice with collagen-induced arthritis.
Lee HS
Arthritis and Rheumatism, 65(7), 1776-1785 (2013)
Minna Rahnasto-Rilla et al.
Chembiochem : a European journal of chemical biology, 17(1), 77-81 (2015-11-27)
Sirtuin 6 (SIRT6) is an NAD+-dependent histone deacetylase enzyme that is involved in multiple molecular pathways related to aging. Initially, it was reported that SIRT6 selectively deacetylated H3K9Ac and H3K56Ac; however, it has more recently been shown to preferentially hydrolyze long-chain

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