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SRP0166

Sigma-Aldrich

DNMT3B/DNMT3L Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

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Synonym(s):
DNA (cytosine-5-)-methyltransferase 3b, ICF, M.HsaIIIB
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

27 kDa (DNMT3L)
59 kDa (DNMT3B)

packaging

pkg of 10 μg

concentration

>0.02 mg/mL

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... DNMT3B(1789)

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This Item
MABE305SRP0396SRP0128
DNMT3a Active human recombinant, expressed in baculovirus infected insect cells

Sigma-Aldrich

SRP0128

DNMT3a Active human

recombinant

expressed in baculovirus infected insect cells

recombinant

-

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected insect cells

assay

≥90% (SDS-PAGE)

assay

-

assay

≥90% (SDS-PAGE)

assay

-

form

aqueous solution

form

-

form

aqueous solution

form

aqueous solution

mol wt

27 kDa (DNMT3L), 59 kDa (DNMT3B)

mol wt

-

mol wt

34 kDa (MLL1), 52 kDa (DNMT3L)

mol wt

128 kDa

packaging

pkg of 10 μg

packaging

antibody small pack of 25 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

General description

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) has two subfamilies-DNMT3B and ΔDNMT3B. These are expressed in many transcript variants. Several single-nucleotide polymorphisms are observed within the promoter region of the DNMT3B gene. The gene encoding this protein is localized on human chromosome 20q11.2. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is part of the DNA methyltransferases family. It has a cysteine-rich region containing a novel-type zinc finger domain. The gene encoding it is localized on human chromosome 21q22.3.

Application

Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) is a de novo methyltransferase which targets the un-methylated 5′-C-phosphate-G-3′ (CpG) sites on the gene promoter. It is responsible for crucial epigenetic mechanisms for the regulation of chromosomal stability and for building a new methylation pattern. The protein is associated with promoter hypermethylation in tumorigenesis and lung cancer. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is a non-catalytic accessory factor which is mainly responsible for the stimulation of DNA methylation. It helps the DNA methylation machinery to be positioned on properly chromatinized DNA regions. The protein also helps to provide uniform methylation patterns. It has an important role in spermatogenesis and mutations in the DNMT3L gene might be associated with azoospermia susceptibility.

Physical form

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCL, 20% glycerol, 4 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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? DNMT3B4-del Contributes to Aberrant DNA Methylation Patterns in Lung Tumorigenesis
Mark Z. Ma
EBioMedicine, 2(10), 1340-1350 (2015)
Associations of DNMT3B -149C>T and -2437T>A polymorphisms and lung cancer risk in Chinese population
Min Gao
World Journal of Surgical Oncology, 14, 293-293 (2016)
The CpG Island Encompassing the Promoter and First Exon of Human DNMT3L Gene Is a PcG/TrX Response Element (PRE)
Amitava Basu
PLoS ONE, 9(4), e93561-e93561 (2014)
DNA methyltransferase 3B -149C/T
polymorphism and the risk of laryngeal
squamous cell carcinoma: a case-control
study
X. M. Zhang
Genetics and molecular research : GMR, 14(4), 12866-12871 (2015)
Polymorphism of DNA Methyltransferase 3b and Association with Development and Prognosis in Gastric Cancer
Chuan Wang
PLoS ONE, 10(8), e0134059-e0134059 (2015)

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