The BCL-2 TR-FRET Assay Kit (BPS Bioscience #50222) was utilized to produce the activity data for Product No. SRP0186, Bcl-2 Active human.
SRP0186
Bcl-2 Active human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym(s):
Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell leukemia/lymphoma 2
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
100 μg | Usually ships in 2 weeks. | $721.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
General description
B-cell lymphoma 2 (BCL-2) is an anti-apoptotic and target gene of nuclear factor-κB (NF-κB). The protein is expressed in the nuclear envelope, mitochondria and endoplasmic reticulum. The gene encoding BCL-2 is localized on human chromosome 18q21.[1][2] Human Bcl-2 (amino-acids 1-211) (GenBank Accession No. P10415) with C-terminal His tag, MW = 27 kDa, expressed in an E. coli expression system.
Application
Useful for the study of apoptosis, screening inhibitors and selectivity profiling.
Biochem/physiol Actions
B-cell lymphoma 2 (BCL-2) inhibits apoptosis in cells and has been shown to be upregulated in adenomas.[1]
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
1 of 1
This Item | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, ≥80% (SDS-PAGE) | description affinity isolated antibody, buffered aqueous solution | description recombinant, expressed in proprietary host, clone SP66, affinity isolated antibody | description clone Bcl-2/100 |
| assay ≥80% (SDS-PAGE) | assay - | assay - | assay - |
| biological source human | biological source rabbit | biological source rabbit | biological source mouse |
| recombinant expressed in E. coli | recombinant - | recombinant expressed in proprietary host | recombinant - |
| form aqueous solution | form buffered aqueous solution | form - | form buffered aqueous solution |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
| storage temp. −70°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
How is the activity testing conducted for Product No. SRP0186, Bcl-2 Active human?
1 answer-
Helpful?
-
-
How can one determine the protein concentration for Product No. SRP0186, Bcl-2 Active human?
1 answer-
The Bradford assay with BSA standard is used to determine the protein concentration for Product No. SRP0186, Bcl-2 Active human.
Helpful?
-