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Key Documents

SRP0186

Sigma-Aldrich

Bcl-2 Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell leukemia/lymphoma 2

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100 μG
$672.00

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100 μG
$672.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

$672.00

Usually ships in 2 weeks.
Request a Bulk Order

biological source

human

recombinant

expressed in E. coli

assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

27 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

P10415

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... BCL2(596)

General description

B-cell lymphoma 2 (BCL-2) is an anti-apoptotic and target gene of nuclear factor-κB (NF-κB). The protein is expressed in the nuclear envelope, mitochondria and endoplasmic reticulum. The gene encoding BCL-2 is localized on human chromosome 18q21.[1][2][3] Human Bcl-2 (amino-acids 1-211) (GenBank Accession No. P10415) with C-terminal His tag, MW = 27 kDa, expressed in an E. coli expression system.

Application

Useful for the study of apoptosis, screening inhibitors and selectivity profiling.[4]

Biochem/physiol Actions

B-cell lymphoma 2 (BCL-2) inhibits apoptosis in cells and has been shown to be upregulated in adenomas.[1][3]

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
5027291006
5027240325
50272111111
50272002000
5027202000

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Rr Suzy Indharty
Asian journal of neurosurgery, 8(2), 83-89 (2013-09-21)
Traumatic brain injury (TBI) is one of the major causes of death and disability. Apoptosis after TBI contributes significantly to the final extent of tissue damage. The Bcl-2 family proteins are important apoptosis modulators which increased in injured neurons. Bcl-2
Maria Lauda Tomasi et al.
Oncotarget, 6(35), 37706-37723 (2015-09-30)
Ubiquitin-conjugating enzyme 9 (Ubc9) is required for sumoylation and inhibits apoptosis via Bcl-2 by unknown mechanism. Methionine adenosyltransferase 2A (MAT2A) encodes for MATα2, the catalytic subunit of the MATII isoenzyme that synthesizes S-adenosylmethionine (SAMe). Ubc9, Bcl-2 and MAT2A expression are
Onursal Onen et al.
Sensors (Basel, Switzerland), 12(9), 12317-12328 (2012-11-01)
Changes in mass loading on the surface of acoustic biosensors result in output frequency shifts which provide precise measurements of analytes. Therefore, to detect a particular biomarker, the sensor delay path must be judiciously designed to maximize sensitivity and specificity.
Christelle Villeneuve et al.
Molecular biology of the cell, 22(7), 999-1013 (2011-02-04)
Although the anti-apoptotic activity of Bcl-2 has been extensively studied, its mode of action remains incompletely understood. Deciphering the network of Bcl-2 interacting factors is necessary to better understand the key function of Bcl-2 in apoptosis initiation. To identify novel
N Heidari et al.
Cell death & disease, 1, e76-e76 (2011-03-03)
Glucocorticoids (GCs) are common components of many chemotherapeutic regimens for lymphoid malignancies including acute lymphoblastic leukemia (ALL). The BCL-2 family has an essential role in regulating GC-induced cell death. Here we show that downregulation of antiapoptotic BCL-2 family proteins, especially
View All Related Papers

Questions

1–2 of 2 Questions  

  1. How can one determine the protein concentration for Product No. SRP0186, Bcl-2 Active human?

    1 answer

    1. The Bradford assay with BSA standard is used to determine the protein concentration for Product No. SRP0186, Bcl-2 Active human.

      Helpful?

  2. How is the activity testing conducted for Product No. SRP0186, Bcl-2 Active human?

    1 answer

    1. The BCL-2 TR-FRET Assay Kit (BPS Bioscience #50222) was utilized to produce the activity data for Product No. SRP0186, Bcl-2 Active human.

      Helpful?

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