SRP0207

PEST Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(s): Lver regeneration-related protein LRRG084, PEST proteolytic signal containing nuclear protein
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 61.4 kDa
• packaging: pkg of 20 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_002835
• UniProt accession no.: Q8WW12
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PCNP(57092)

Description

General description

Protein tyrosine phosphatase, Catalytic domain, (amino acids 2-300) (Gene bank accession number NM_002835) with N-terminal GST tag, MW = 61.4 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.