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SRP0216

Sigma-Aldrich

SHP-1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

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Synonym(s):
HCP, Hematopoietic cell phosphatase, PTP-1C, PTPN6, SHP-1L
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

95 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

1 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTPN6(5777)

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vibrant-m

SRP0216

SHP-1 Active human

vibrant-m

SRP0224

PTPsigma/PTPRS Active human

vibrant-m

SRP0211

PTP-D2/PTPN14 Active human

vibrant-m

SRP0215

PTP1B full length Active human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

biological source

human

biological source

human

biological source

human

biological source

human

assay

≥90% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

≥55% (SDS-PAGE)

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

(Tightly closed), avoid repeated freeze/thaw cycles

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Protein tyrosine phosphatase, full length, (Gene bank accession number NM_080549) with N-terminal GST tag, MW = 95 kDa, expressed in an E. coli expression system.
The Src homology region 2 domain-containing phosphatase-1 (SHP1)/protein tyrosine phosphatase, non-receptor type 6 (PTPN6) is a 68 kDa protein present in the cytoplasm. It is mapped to human chromosome 12p13.

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Biochem/physiol Actions

In mammary epithelial cells, protein tyrosine phosphatase, non-receptor type 6 (PTPN6) controls EGFR (epidermal growth factor receptor) signaling. It participates in the JAK/STAT (janus kinase/signal transducers and activators of transcription) signaling pathway. PTPN6 regulates cytokine receptors like interleukin- 3 (IL-3) and erythropoietin.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Shp1 positively regulates EGFR signaling by controlling EGFR protein expression in mammary epithelial cells
Yuan T, et al.
Biochemical and Biophysical Research Communications, 488(3), 439-444 (2017)
Promoter methylation attenuates SHP1 expression and function in patients with primary central nervous system lymphoma
Liu J, et al.
Oncology Reports, 37(2), 887-894 (2017)
Priya Saju et al.
Nature microbiology, 1, 16026-16026 (2016-08-31)
Most if not all gastric cancers are associated with chronic infection of the stomach mucosa with Helicobacter pylori cagA-positive strains(1-4). Approximately 10% of gastric cancers also harbour Epstein-Barr virus (EBV) in the cancer cells(5,6). Following delivery into gastric epithelial cells
Wei Luo et al.
Nature immunology, 20(6), 736-746 (2019-04-24)
B cell antigen receptor (BCR) and CD40 signaling are rewired in germinal center (GC) B cells (GCBCs) to optimize selection for high-affinity B cells. In GCBC, BCR signals are constrained, but the mechanisms are not well understood. Here we describe
A Beghini et al.
Human molecular genetics, 9(15), 2297-2304 (2000-09-26)
The SH2 domain-containing tyrosine phosphatase PTPN6 (SHP-1, PTP1C, HCP) is a 68 kDa cytoplasmic protein primarily expressed in hematopoietic cell development, proliferation and receptor-mediated mitogenic signaling pathways. By means of direct dephosphorylation, it down-regulates a broad spectrum of growth-promoting receptors

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