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SRP0230

Sigma-Aldrich

XIAP Active human

recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)

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Synonym(s):
Baculoviral IAP repeat-containing protein 4 (BIRC4), E3 ubiquitin-protein ligase XIAP (MIHA), IAP-like protein (ILP1), Inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥65% (SDS-PAGE)

form

aqueous solution

mol wt

57 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... XIAP(331)

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This Item
PRS3331SAB4301454ZRB1709
XIAP Active human recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)

Sigma-Aldrich

SRP0230

XIAP Active human

recombinant

expressed in baculovirus infected insect cells

recombinant

-

recombinant

-

recombinant

expressed in HEK 293 cells

assay

≥65% (SDS-PAGE)

assay

-

assay

-

assay

-

form

aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized

mol wt

57 kDa

mol wt

predicted mol wt 55 kDa

mol wt

57 kDa

mol wt

calculated mol wt 56.69 kDa, observed mol wt ~52 kDa

storage condition

avoid repeated freeze/thaw cycles

storage condition

-

storage condition

-

storage condition

-

General description

XIAP (X-linked inhibitor of apoptosis) is an anti-apoptotic protein belonging to the inhibitor of apoptosis protein (IAP) family, an evolutionary conserved protein family. XIAP contains three tandem copies of BIR (baculovirusIAP repeat) domain, which is composed of ∼70 residues. IAP family members also contain a RING (really interesting new gene) zinc finger domain in their C-terminals.

Application

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

XIAP (X-linked inhibitor of apoptosis) is the most potent caspase-interacting protein and inhibits both intrinsic and extrinsic pathways of apoptosis. Its up-regulation in tumor cells is linked with resistance to chemotherapy. In inflammatory breast cancer (IBC) this protein is linked with resistance to antibody-dependent cellular cytotoxicity (ADCC) driven by cetuximab and trastuzumab. Embelin, an inhibitor of XIAP, functions as an anti-tumor agent in myeloma cell line (HL-60) by suppressing the expression of XIAP. Elevated expression of XIAP in rectal cancer is associated with chemotherapy resistance.

Unit Definition

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol, and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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X-linked inhibitor of apoptosis protein mediates tumor cell resistance to antibody-dependent cellular cytotoxicity.
Evans MK et al
Cell Death & Disease, 7, e2073-e2073 (2016)
Synergism between NF-kappa B inhibitor, celastrol, and XIAP inhibitor, embelin, in an acute myeloid leukemia cell line, HL-60.
Pazhang Y et al
Journal of Cancer Research and Therapeutics, 12(1), 155-160 (2016)
Jun Zhou et al.
Cancer immunology, immunotherapy : CII, 68(8), 1331-1340 (2019-07-19)
Expression of inhibitors of apoptosis protein (IAP) family members is associated with poor prognosis in cancer patients. Immunity to ML-IAP (livin) and survivin has been well studied in patients with a variety of tumors. XIAP, the most potent inhibitor of
A single BIR domain of XIAP sufficient for inhibiting caspases.
Takahashi R et al
The Journal of Biological Chemistry, 273(14), 7787-7790 (1998)
Arun Murali et al.
Cell death & disease, 8(6), e2900-e2900 (2017-07-01)
Rho GTPases control fundamental cellular processes and Cdc42 is a well-studied member of the family that controls filopodia formation and cell migration. Although the regulation of Cdc42 activity by nucleotide binding is well documented, the mechanisms driving its proteostasis are

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