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SRP0232

Sigma-Aldrich

p85a Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

Synonym(s):
GRB1, PI3-kinase regulatory subunit alpha, PIK3R1, Phosphoinositide-3-kinase, regulatory subunit 1 (α)
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

88 kDa

packaging

pkg of 100 μg

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PIK3R1(5295)

General description

Human p85α ( GenBank Accession No.XM_043865), full length with N-terminal His tag, MW = 88 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of p85alpha biochemical function.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificate of Analysis

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Certificate of Origin

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