SRP0258

PDE1B Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonym(s): Calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase, Cam-PDE 63 kDa, PDES1B, Phosphodiesterase 1B
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥70% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥2000 units/μg
• mol wt: 87 kDa
• packaging: pkg of 10 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_000924
• UniProt accession no.: Q01064
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PDE1B(5153)

Description

General description

Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.