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SRP0264

Sigma-Aldrich

PDE4B2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

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Synonym(s):
DPDE4, PDE4B, Phosphodiesterase 4B, cAMP-specific, Phosphodiesterase 4B2

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

91.2 kDa

packaging

pkg of 5 μg

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PDE4B(24626)

General description

Human PDE4B2 (GenBank Accession No. NM_001037339), full length, with N-terminal GST tag, MW = 91.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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