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SRP0309

Sigma-Aldrich

ATAT1 human

recombinant, expressed in E. coli, ≥55% (SDS-PAGE)

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Synonym(s):
MEC17, TAT, alpha tubulin acetyltransferase 1
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥55% (SDS-PAGE)

form

aqueous solution

mol wt

52 kDa

packaging

pkg of 50 μg

NCBI accession no.

NM_001031722 (var1)

UniProt accession no.

Q5SQI0

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... ATAT1(79969)

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Aldose Reductase human recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Sigma-Aldrich

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Aldose Reductase human

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in insect cells

recombinant

expressed in E. coli

assay

≥55% (SDS-PAGE)

assay

-

assay

≥70% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

aqueous solution

form

buffered aqueous solution

form

frozen liquid

form

liquid

mol wt

52 kDa

mol wt

46 kDa

mol wt

~89.1 kDa

mol wt

35.8 kDa (316 aa, 1-316 aa)

packaging

pkg of 50 μg

packaging

-

packaging

pkg of 10 μg

packaging

pkg of 100 μg

General description

Human α tubulin acetyltransferase 1, or ATAT1, (GenBank Accession No. NM_001031722 (var1)), amino acids 2 – 224 with N-terminal GST-tag, MW=52 kDa, expressed in an E. coli expression system.
The αTAT1 (α tubulin acetyltransferase 1) protein sequence of αTAT1 is highly conserved among eukaryotes. αTAT1 is a member of Gcn5 (general control non-depressible 5)-related N-acetyltransferase superfamily and is mostly present in ciliated organisms. It has also been identified in Tetrahymena, nematodes, zebrafish and mice.

Biochem/physiol Actions

ATAT1 (α tubulin acetyltransferase 1) catalyses the acetylation of lysine 40 of α-tubulin and microtubule. ATAT1 expression is essential for microtubule stabilization and mediates cilium formation. Upart from acetylation, αTAT1 interacts with doublecortin (a microtubule associated protein) to degrade microtubules that are not aligned with acetyltransferase activity. αTAT1 regulates the Wnt1 signalling pathway via β-catenin and promotes tumorigenesis. Overexpression of the ATAT1 gene is observed in colon cancer and is known to contribute to its progression.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Structural basis of cofactor-mediated stabilization and substrate recognition of the ?-tubulin acetyltransferase ?TAT1.
Yuzawa S, et al.
The Biochemical Journal, 467(1), 103-113 (2015)
Microtubules acquire resistance from mechanical breakage through intralumenal acetylation.
Xu Z, et al.
Science, 356(6335), 328-332 (2017)
R A Lloyd et al.
Analytical biochemistry, 216(1), 42-46 (1994-01-01)
An assay for alpha-tubulin acetyltransferase (TAT) activity based on affinity isolation of labeled acetylated alpha-tubulin was developed for use with crude subcellular fractions. Microtubules were polymerized and immobilized on an anti-alpha-tubulin-agarose and then incubated with the subcellular fraction and [3H]acetyl-coenzyme
Genetic disruption of tubulin acetyltransferase, ?TAT1, inhibits proliferation and invasion of colon cancer cells through decreases in Wnt1/?-catenin signaling.
Oh S, et al.
Biochemical and Biophysical Research Communications, 482(1), Aug-A14 (2017)
Tubulin acetylation: responsible enzymes, biological functions and human diseases.
Li L and Yang X J
Cellular and Molecular Life Sciences, 72(22), 4237-4255 (2015)
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