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SRP0320

Sigma-Aldrich

JMJD2b active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

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Synonym(s):
KDM4B, lysine (K)-specific demethylase 4B
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

83 kDa

packaging

pkg of 100 μg

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... KDM4B(23030)

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JMJD2b active human recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE)

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SRP0320

JMJD2b active human

JMJD2B Active human recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)

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SRP0169

JMJD2B Active human

JMJD2D active human recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)

Sigma-Aldrich

SRP0321

JMJD2D active human

JMJD1A human recombinant, expressed in baculovirus infected Sf9 cells, ≥40% (SDS-PAGE)

Sigma-Aldrich

SRP0318

JMJD1A human

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥70% (SDS-PAGE)

assay

≥40% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

≥40% (SDS-PAGE)

form

aqueous solution

form

aqueous solution

form

aqueous solution

form

aqueous solution

mol wt

83 kDa

mol wt

57 kDa

mol wt

67 kDa

mol wt

148.2 kDa

packaging

pkg of 100 μg

packaging

pkg of 100 μg

packaging

pkg of 100 μg

packaging

pkg of 20 μg

General description

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), amino acids 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Specificity

0.32 pmole/min/μg Assay conditions: 10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM a-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin conjugated secondary antibody followed by Alpha Screening detection.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol.

Storage and Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Barna D Fodor et al.
Genes & development, 20(12), 1557-1562 (2006-06-02)
Histone lysine trimethyl states represent some of the most robust epigenetic modifications in eukaryotic chromatin. Using a candidate approach, we identified the subgroup of murine Jmjd2 proteins to antagonize H3K9me3 at pericentric heterochromatin. H3K27me3 and H4K20me3 marks are not impaired
Sophie Beyer et al.
The Journal of biological chemistry, 283(52), 36542-36552 (2008-11-06)
Posttranslational histone modifications serve to store epigenetic information and control both nucleosome assembly and recruitment of non-histone proteins. Histone methylation occurs on arginine and lysine residues and is involved in the regulation of gene transcription. A dynamic control of these

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