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SRP0736

Sigma-Aldrich

PP2A ALPHA/PPP2R1A Complex, Active

recombinant

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UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in baculovirus infected Sf9 cells

assay

70-95% (SDS-PAGE)

form

frozen liquid

specific activity

3400-4600 nmol/min·mg (BIOMOL Green)

mol wt

~38 kDa
~65 kDa

concentration

0.1 μg/μL

accession no.

NM_002715
NM_014225

shipped in

dry ice

storage temp.

−70°C

Gene Information

Application

Useful for Phosphatase assay and Western blot.

Biochem/physiol Actions

Recombinant full length human PP2Aalpha and human PPP2R1A were expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A (1). Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2).

Reconstitution

Formulated in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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