The supplied amount of protein is 10 μg. The concentration of the protein is approximately 0.1 μg/μL, but may vary slightly from lot to lot. This minimum purity is 70% as determined by densitometry. The PP2Aa molecular weight is approximately 38 kDa and the PP2R1A molecular weight is approximately 65 kDa as shown in the image below:
SRP0736
PP2A ALPHA/PPP2R1A Complex, Active
recombinant
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10 μG
$542.00
$542.00
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
frozen liquid
Assay:
70-95% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
~38 kDa, ~65 kDa
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recombinant
expressed in baculovirus infected Sf9 cells
assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
3400-4600 nmol/min·mg (BIOMOL Green)
mol wt
~38 kDa, ~65 kDa
concentration
0.1 μg/μL
accession no.
NM_002715, NM_014225
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PP2A(5524), PPP2R1A(5518)
1 of 4
This Item | |||
|---|---|---|---|
| assay 70-95% (SDS-PAGE) | assay 70-95% (SDS-PAGE) | assay 70-95% (SDS-PAGE) | assay 70-95% (SDS-PAGE) |
| recombinant expressed in baculovirus infected Sf9 cells | recombinant expressed in baculovirus infected Sf9 cells | recombinant expressed in baculovirus infected Sf9 cells | recombinant expressed in baculovirus infected Sf9 cells |
| concentration 0.1 μg/μL | concentration 0.1 μg/μL | concentration 0.1 μg/μL | concentration 0.1 μg/μL |
| mol wt ~38 kDa, ~65 kDa | mol wt ~73 kDa | mol wt ~64 kDa (Cyclin Y), ~88 kDa (PCTK2) | mol wt ~64 kDa (Cyclin Y), ~76 kDa (PCTK3) |
| form frozen liquid | form frozen liquid | form frozen liquid | form frozen liquid |
| Gene Information human ... PP2A(5524), PPP2R1A(5518) | Gene Information human ... PIP4K2B(8396) | Gene Information human ... CCNY(219771), CDK17(5128) | Gene Information human ... CCNY(219771), CDK18(5129) |
Application
Useful for Phosphatase assay and Western blot.
Biochem/physiol Actions
Recombinant full length human PP2Aalpha and human PPP2R1A were expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A (1). Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2).
Preparation Note
Formulated in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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