MilliporeSigma
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SRP2005

Sigma-Aldrich

TFIIE β, p34 human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym(s):
FE, TF2E2, TFIIE-B
NACRES:
NA.26

biological source

human

recombinant

expressed in E. coli

assay

≥70% (SDS-PAGE)

form

frozen liquid

mol wt

~35 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

250 μg/mL

technique(s)

western blot: suitable

color

clear colorless

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... GTF2E2(2961)

Biochem/physiol Actions

The human Transcription Factor IIE (TFIIE) is composed of 56 kDa ( α) and 34 kDa ( β) subunits and is shown to be a heterotetramer. The 56-kDa subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain. TFIIE binds to RNA polymerase II in solution and joins the pre-initiation complex probably concomitant with RNA polymerase II and TFIIF.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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