SRP2120

RNA Polymerase II Peptide

≥70% (SDS-PAGE), human recombinant, expressed in E. coli, GST tagged

• Synonym(s): POLRA, RPB1, RPO2, RPOL2, hRPB220
• UNSPSC Code: 12352202
• NACRES: NA.26

Properties

• Product Name: RNA Polymerase II, C-terminal domain, GST tagged human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
• biological source: human
• recombinant: expressed in E. coli
• assay: ≥70% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~68.1 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• color: clear colorless
• NCBI accession no.: NM_000937
• UniProt accession no.: P24928
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... POLR2A(5430)

Description

Biochem/physiol Actions

The CTD works as a binding scaffold for nuclear factors through its phosphorylation. It is suggested to be also involved in chromatin structure modification, DNA damage/repair, protein degradation and synthesis, RNA degradation, snRNA (small nuclear RNA) modification, and snoRNP (small nucleolar ribonucleoprotein) biogenesis.[1]

The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms. There are two forms of RNA pol II in vivo: IIO, which is extensively phosphorylated at the CTD, and IIA, which is not phosphorylated. The IIA form preferentially enters the pre-initiation complex (PIC), whereas IIO is found in the elongation complex. The kinase activity of TFIIH can mediate CTD phosphorylation, although other kinases, including Cdc2, Ctk1, the Srb10-Srb11 kinase-cyclin pair, and P-TEFb, have also been implicated in CTD phosphorylation. A phosphatase responsible for the dephosphorylation of the CTD has also been identified. CTD phosphatase activity is regulated by TFIIB and TFIIF. The CTD has also been implicated in pre-mRNA processing, most likely functioning as a platform for the recruitment and assembly of factors involved in pre-mRNA processing.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable