SRP3015

Beta NGF human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), suitable for cell culture

• Synonym(s): beta-Nerve Growth Factor
• NACRES: NA.32
• UNSPSC Code: 12352202

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE)
• form: lyophilized
• potency: <1.0 ng/mL ED₅₀
• mol wt: (Noncovalent Homodimer, 13.6 kDa (121 amino acids))
• packaging: pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• UniProt accession no.: P01138
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... NGF(4803)

Description

General description

β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (240 total amino acid residues). The three disulfide bonds are required for biological activity.

Application

β NGF human has been used in immunohistochemistry.[1]

Biochem/physiol Actions

β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The functional form of human b-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (240 total amino acid residues). The three disulfide bonds are required for biological activity.

Physical form

Lyophilized from a 0.2 μm filtered solution containing0.1% Trifluoroacetic Acid (TFA)

Preparation Note

Sterile water at 0.1 mg/mLCentrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.

Other Notes

SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable