SRP3029

Sigma-Aldrich

EG-VEGF human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
PROK1, Prokineticin 1, Endocrine Gland-derived Vascular Endothelial Growth Factor
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

9.6 kDa

packaging

pkg of 20 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PROK1(84432)

General description

EG-VEGF (endocrine gland-derived vascular endothelial growth factor) belongs to the prokineticin family. It is mainly expressed in the steroidogenic glands (ovary, testis, adrenal cortex and placenta). EG-VEGF is a small, secreted peptide and works as a tissue-specific pro-angiogenic mitogen and chemotactic agent. The gene is mapped to human chromosome 1p13.
Recombinant human EG-VEGF is a 9.6kDa protein consisting of 86 amino acid residues and ten cysteine residues that potentially form five pairs of intra-molecular disulfide bonds.

Biochem/physiol Actions

EG-VEGF (endocrine gland-derived vascular endothelial growth factor) is involved in angiogenesis, inflammatory responses and hematopoiesis. It also participates in female reproduction. EG-VEGF suppresses trophoblast invasion in pregnancy. Changes in the activity of this protein are associated with gestational complication, such as gestational trophoblastic diseases, repeated pregnancy losses, preeclampsia and intrauterine fetal growth restriction. EG-VEGF interacts with G protein-coupled receptors, prokineticin receptor 1 (PKR1) and prokineticin receptor 2 (PKR2). It is also involved in the pathology of Leydig-cell-tumors, papillary thyroid cancer, neuroblastoma, prostate cancer, gastrointestinal tumors, pancreatic ductal adenocarcinoma, Merkel cell carcinoma and multiple myeloma.

Sequence

MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPALPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Takanori Goi et al.
Oncotarget, 6(8), 6053-6061 (2015-03-20)
Hematogenous metastasis, mainly hepatic metastasis, is a frequent metastatic mode in colorectal cancer involving angiogenic growth factors. Two angiogenic growth factors, in particular, Vascular endothelial growth factor (VEGF) and Prokineticin1(PROK1), are considered to have an important role in hematogenous metastasis...
Hirschsprung's disease as a model of complex genetic etiology.
Borrego S, et al.
Histology and Histopathology, 28, 1117-1117 (2013)
A Common Variant of PROK1 (V67I) Acts as a Genetic Modifier in Early Human Pregnancy through Down-Regulation of Gene Expression.
Su MT
International Journal of Molecular Sciences, 17(2), 17(2)-17(2) (2016)
A Common Variant of PROK1 (V67I) Acts as a Genetic Modifier in Early Human Pregnancy through Down-Regulation of Gene Expression.
Su MT, et al.
International Journal of Molecular Sciences, 17, E162-E162 (2016)
Role of Endocrine Gland-Derived Vascular Endothelial Growth Factor (EG-VEGF) and Its Receptors in Adrenocortical Tumors.
Heck D, et al.
Hormones & Cancer, 6, 225-225 (2015)

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