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SRP3033

Sigma-Aldrich

Epiregulin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):
EREG

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

<0.2 ng/mL

mol wt

5.6 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EREG(2069)

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This Item
SRP3027SRP3029E8780
Epiregulin human recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), &#8805;98% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3033

Epiregulin human

Sigma-Aldrich

Sigma-Aldrich

SRP3027

EGF human

EG-VEGF human recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), &#8805;98% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3029

EG-VEGF human

SAFC

SAFC

E8780

Epiregulin from mouse

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized powder

mol wt

5.6 kDa

mol wt

6.2 kDa

mol wt

9.6 kDa

mol wt

predicted mol wt ~5.5 kDa

packaging

pkg of 25 μg

packaging

pkg of 500 μg

packaging

pkg of 20 μg

packaging

-

General description

EREG (epiregulin) is mainly known as a ligand of EGFR (epidermal growth factor receptor) and ErbB-4 (receptor tyrosine-protein kinase). It induces tyrosine phosphorylation of EGFR, ErbB-2, ErbB-3 and ErbB-4. The mechanism of action involves interaction with EGFR, dimerization of EGFR and induction of autophosphorylation. The EREG gene is mapped to human chromosome 4q13.
Recombinant human Epiregulin is a 5.6kDa monomeric protein, containing 50 amino residues, which corresponds to the mature secreted Epiregulin sequence.

Application

Epiregulin human has been added in the culture medium to study the effect of epiregulin on epithelial invasion.
Epiregulin human has been used as a supplement in media 199 to stimulate human epidermal keratinocytes. It has also been used to treat colon cancer cells to test its effect on cell migration.

Biochem/physiol Actions

EREG (epiregulin)-mediated EGFR (epidermal growth factor receptor) phosphorylation is responsible for the activation of various pathways, including the mitogen-activated protein kinase (MAPK), phosphoinositide 3-kinase (PI3K) and STAT5 (signal transducer and activator of transcription 5) responses. These pathways are involved in proliferation, cell survival, and angiogenesis. EREG is upregulated in colon, breast and ovarian cancers.

Sequence

MVAQVSITKC SSDMNGYCLH GQCIYLVDMS QNYCRCEVGY TGVRCEHFFL

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Keratinocytes can differentiate into eccrine sweat ducts in vitro: involvement of epidermal growth factor and fetal bovine serum.
Shikiji T, et al.
Journal of Dermatological Science, 33, 141-141 (2003)
Epiregulin Recognition Mechanisms by Anti-epiregulin Antibody 9E5: STRUCTURAL, FUNCTIONAL, AND MOLECULAR DYNAMICS SIMULATION ANALYSES.
Kado Y, et al.
The Journal of Biological Chemistry, 291, 2319-2319 (2016)
Chanat Kumtornrut et al.
Journal of dermatological science, 93(3), 150-158 (2019-02-23)
The main pathogenesis of acne vulgaris is increase in sebum production and abnormal keratinization of the hair infundibulum. The androgens are involved in acne pathogenesis by modulating sebaceous glands to enhance sebum production. However, the molecular mechanisms of abnormal keratinization
Yuji Kado et al.
The Journal of biological chemistry, 291(5), 2319-2330 (2015-12-03)
Epiregulin (EPR) is a ligand of the epidermal growth factor (EGF) family that upon binding to its epidermal growth factor receptor (EGFR) stimulates proliferative signaling, especially in colon cancer cells. Here, we describe the three-dimensional structure of the EPR antibody
The human obesity gene map: the 2005 update.
Rankinen T, et al.
Obesity (Silver Spring, Md.), 14, 529-529 (2006)

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