SRP3033

Sigma-Aldrich

Epiregulin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
EREG
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

<0.2 ng/mL

mol wt

5.6 kDa

packaging

pkg of 25 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EREG(2069)

General description

EREG (epiregulin) is mainly known as a ligand of EGFR (epidermal growth factor receptor) and ErbB-4 (receptor tyrosine-protein kinase). It induces tyrosine phosphorylation of EGFR, ErbB-2, ErbB-3 and ErbB-4. The mechanism of action involves interaction with EGFR, dimerization of EGFR and induction of autophosphorylation. The EREG gene is mapped to human chromosome 4q13.
Recombinant human Epiregulin is a 5.6kDa monomeric protein, containing 50 amino residues, which corresponds to the mature secreted Epiregulin sequence.

Application

Epiregulin human has been used as a supplement in media 199 to stimulate human epidermal keratinocytes. It has also been used to treat colon cancer cells to test its effect on cell migration.
Epiregulin human has been added in the culture medium to study the effect of epiregulin on epithelial invasion.

Biochem/physiol Actions

EREG (epiregulin)-mediated EGFR (epidermal growth factor receptor) phosphorylation is responsible for the activation of various pathways, including the mitogen-activated protein kinase (MAPK), phosphoinositide 3-kinase (PI3K) and STAT5 (signal transducer and activator of transcription 5) responses. These pathways are involved in proliferation, cell survival, and angiogenesis. EREG is upregulated in colon, breast and ovarian cancers.

Sequence

MVAQVSITKC SSDMNGYCLH GQCIYLVDMS QNYCRCEVGY TGVRCEHFFL

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Serum levels of hepatocyte growth factor and epiregulin are associated with the prognosis on anti-EGFR antibody treatment in KRAS wild-type metastatic colorectal cancer.
Takahashi N, et al.
British Journal of Cancer, 110, 2716-2716 (2014)
The human obesity gene map: the 2005 update.
Rankinen T, et al.
Obesity (Silver Spring, Md.), 14, 529-529 (2006)
Chanat Kumtornrut et al.
Journal of dermatological science, 93(3), 150-158 (2019-02-23)
The main pathogenesis of acne vulgaris is increase in sebum production and abnormal keratinization of the hair infundibulum. The androgens are involved in acne pathogenesis by modulating sebaceous glands to enhance sebum production. However, the molecular mechanisms of abnormal keratinization...
Y Shirakata et al.
The Journal of biological chemistry, 275(8), 5748-5753 (2000-02-22)
Epiregulin is a new member of the epidermal growth factor (EGF) family purified from conditioned medium of NIH-3T3 clone T7. Some EGF family growth factors play essential roles in human keratinocytes in an autocrine manner. We show here that epiregulin...
Epiregulin Recognition Mechanisms by Anti-epiregulin Antibody 9E5: STRUCTURAL, FUNCTIONAL, AND MOLECULAR DYNAMICS SIMULATION ANALYSES.
Kado Y, et al.
The Journal of Biological Chemistry, 291, 2319-2319 (2016)

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