SRP3040

Sigma-Aldrich

FGF-9 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):
GAF (Glia-activating factor), Fibroblast Growth Factor-9, HBGF-9
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

≥2x10^6 units/mg

mol wt

23.4 kDa

packaging

pkg of 20 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FGF9(2254)

General description

FGF9 (fibroblast growth factor 9) is a member of the FGF family, which consists of a minimum of 24 members. This protein is highly conserved across species, and the gene is localized to human chromosome 13q11-q12. In embryos, FGF9 mRNA has a ubiquitous expression pattern. In adults, this protein has a limited range of expression in normal conditions, and expression is usually restricted to brain, kidney and uterus.
Recombinant human FGF-9 is a 23.4kDa protein consisting of 207 amino acid residues.

Biochem/physiol Actions

FGF9 (fibroblast growth factor 9) is expressed in the epithelium and mesothelium of developing lung in the pseudoglandular stage. In developing lungs, it regulates the branching of epithelium, proliferation of mesenchymal cells, and expansion of the lungs. It is overexpressed in patients with idiopathic pulmonary fibrosis (IPF), in regions demonstrating active cell hyperplasia, metaplasia and fibrosis. Thus, it might facilitate the pathogenesis of IPF. The expression of this protein is up-regulated in colon cancer, which is an outcome of hypoxia-induced translational activation.

Sequence

MAPLGEVGNY FGVQDAVPFG NVPVLPVDSP VLLSDHLGQS EAGGLPRGPA VTDLDHLKGI LRRRQLYCRT GFHLEIFPNG TIQGTRKDHS RFGILEFISI AVGLVSIRGV DSGLYLGMNE KGELYGSEKL TQECVFREQF EENWYNTYSS NLYKHVDTGR RYYVALNKDG TPREGTRTKR HQKFTHFLPR PVDPDKVPEL YKDILSQS

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.8 + 50 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
K Harpelunde Poulsen et al.
Human reproduction (Oxford, England), 34(11), 2228-2243 (2019-11-18)
Does experimental manipulation of fibroblast growth factor 9 (FGF9)-signalling in human fetal gonads alter sex-specific gonadal differentiation? Inhibition of FGFR signalling following SU5402 treatment impaired germ cell survival in both sexes and severely altered the developing somatic niche in testes...
Tsung-Ming Chen et al.
Nucleic acids research, 42(5), 2932-2944 (2013-12-18)
Human fibroblast growth factor 9 (FGF9) is a potent mitogen involved in many physiological processes. Although FGF9 messenger RNA (mRNA) is ubiquitously expressed in embryos, FGF9 protein expression is generally low and restricted to a few adult organs. Aberrant expression...
Emily Coffey et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 61(9), 671-679 (2013-06-26)
The fibroblast growth factor (FGF) family of signaling ligands contributes significantly to lung development and maintenance in the adult. FGF9 is involved in control of epithelial branching and mesenchymal proliferation and expansion in developing lungs. However, its activity and expression...
Chao Sun et al.
BMC cancer, 15, 333-333 (2015-05-01)
Cancer-associated fibroblasts (CAFs), which reside around tumor cells, are suggested to play a pivotal role in tumor progression. Here we performed microarray analyses to compare gene expression profiles between CAFs and non-cancerous gastric fibroblasts (NGFs) from a patient with gastric...
Fibroblast growth factors.
Ornitz, D.M., et al.
Genome Biology, 2, 30051-300512 (2001)

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