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SRP3043

Sigma-Aldrich

FGF-basic human

Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

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Synonym(s):
Catabolin, Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Lymphocyte-activating factor (LAF), Mononuclear Cell Factor (MCF)

biological source

human

recombinant

expressed in E. coli

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

≥1x10^7 units/mg

mol wt

17.2 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FGF2(2247)

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FGF-2 human

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FGF-16 human

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FGF-9 human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

mol wt

17.2 kDa

mol wt

~17.2 kDa

mol wt

23.7 kDa

mol wt

23.4 kDa

packaging

pkg of 50 μg

packaging

pkg of 50 μg

packaging

pkg of 25 μg

packaging

pkg of 20 μg

General description

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.

Application

FGF-basic has been used to supplement serum-free DMEM during culture of HeLa cells.

Biochem/physiol Actions

FGF-basic (fibroblast growth factor), also referred to as FGF2, activates the ERK (extracellular signal-regulated kinase) cascade and facilitates self-renewal of hESCs (human embryonic stem cells). FGF2 positively regulates cell survival and/or proliferation. It is found to negatively regulate BMP (bone morphogenetic protein) signaling, a pathway that induces non-neuronal differentiation.

Sequence

AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Physical form

Lyophilized from 5 mM Tris, pH 7.6 + 150mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8° C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 5% Trehalose) and store in working aliquots at -20° C to -80° C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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