SRP3090

Sigma-Aldrich

IL-3 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
HCGF, Multi-CSF, P-cell stimulation factor, MCGF (Mast cell growth factor)
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.1 ng/mL ED50

mol wt

15.0 kDa

packaging

pkg of 10 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL3(3562)

General description

IL3 (interleukin 3) is a haematopoietic growth factor, the gene for which is localized to human chromosome locus 5.
Recombinant human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.

Biochem/physiol Actions

IL3 (interleukin 3) promotes the survival, proliferation and differentiation of haematopoietic progenitor cells. It also functions as an activator of basophils thus, resulting in significant increase in antigen and IgE-mediated release of cytokines and other mediators. Certain variants in this gene confer protection against malaria attack, and thus, this gene might play a role in the pathophysiology of falciparum malaria. SNP (single nucleotide polymorphism) rs40401 in this gene and smoking have a positive correlation with asthma in Japanese women. IL3 is involved in brain development and has a direct influence on the proliferation and survival of neural progenitor cell population, thereby also controlling human brain volume.

Sequence

APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Christian G Meyer et al.
Human molecular genetics, 20(6), 1173-1181 (2011-01-13)
Using segregation analyses, control of malaria parasites has previously been linked to a major gene within the chromosomal region 5q31-33, but also to complex genetic factors in which effects are under substantial age-dependent influence. However, the responsible gene variants have...
Y Miyake et al.
Scandinavian journal of immunology, 79(6), 410-414 (2014-04-02)
Three previous genetic association studies conducted in Korea and China investigated the relationship between IL3 single nucleotide polymorphism (SNP) rs40401 and asthma, but their results were inconsistent. We examined this relationship and the possibility of an interaction between IL3 SNP...
Xiong-jian Luo et al.
PloS one, 7(11), e50375-e50375 (2012-12-12)
One of the most significant evolutionary changes underlying the highly developed cognitive abilities of humans is the greatly enlarged brain volume. In addition to being far greater than in most other species, the volume of the human brain exhibits extensive...
J N Ihle
Chemical immunology, 51, 65-106 (1992-01-01)
Considerable information has accumulated over the past several years relating to the biological activities of IL-3 which supports that concept that IL-3 has a unique capacity to support the proliferation and differentiation of a wide spectrum of hematopoietic cells. The...
Amy H Lin et al.
Journal of virology, 88(17), 10066-10077 (2014-06-27)
We developed a Moloney mouse leukemia virus (MLV)-based retroviral replicating vector (RRV), Toca 511, which has displayed tumor specificity in resected brain tumor material and blood in clinical trials. Here, we investigated the interaction between Toca 511 and human host...

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