SRP3095

IL-5 human

Name: IL-5 human
Brand: SIGMA
Price: 397 USD

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

BCDFII, EDF

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10 μG

$397.00

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

Key Documents

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Product Name

IL-5 human, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.25 ng/mL ED₅₀

mol wt

26.0 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

off-white to yellow

UniProt accession no.

P05113

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL5(3567)

Related Categories

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Growth Factors and Cytokines

Molecular Biology & Functional Genomics

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1 of 4

This Item	SRP3212	SRP3093	SRP3099
Sigma-Aldrich SRP3095 IL-5 human	Sigma-Aldrich SRP3212 IL-5 from mouse	Sigma-Aldrich SRP3093 IL-4 human	Sigma-Aldrich SRP3099 IL-9 human
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
biological source human	biological source mouse	biological source human	biological source human
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)
form lyophilized	form lyophilized	form lyophilized	form lyophilized
mol wt 26.0 kDa	mol wt 26.2 kDa	mol wt 14.9 kDa	mol wt 14.0 kDa

General description

IL-5 is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils. Produced by mast cells, T cells, and eosinophils, IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. Elevated levels of IL-5 lead to Eosinophilia, which may result in the induction of asthma and other allergic diseases. Human and murine IL-5 is cross-species reactive. Recombinant human IL-5 is a 26.0 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains.

Other Notes

MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES

Physical form

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium glycinate, pH 8.5

Biochem/physiol Actions

IL-5 is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils. Recombinant human IL-5 is a 26.0 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0615

090815

060515-A

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Molecular cloning, nucleotide sequence, and expression of the gene encoding human eosinophil differentiation factor (interleukin 5).

H D Campbell et al.

Proceedings of the National Academy of Sciences of the United States of America, 84(19), 6629-6633 (1987-10-01)

The human eosinophil differentiation factor (EDF) gene was cloned from a genomic library in lambda phage EMBL3A by using a murine EDF cDNA clone as a probe. The DNA sequence of a 3.2-kilobase BamHI fragment spanning the gene was determined.

ATP drives eosinophil effector responses through P2 purinergic receptors.

Takehito Kobayashi et al.

Allergology international : official journal of the Japanese Society of Allergology, 64 Suppl, S30-S36 (2015-09-08)

Eosinophils recognize various stimuli, such as cytokines, chemokines, immunoglobulins, complement, and external pathogens, resulting in their accumulation in mucosal tissues and the progression of inflammation. Eosinophils are also involved in innate Th2-type immune responses mediated through endogenous danger signals, including

Interleukin-5, eosinophils, and disease.

C J Sanderson

Blood, 79(12), 3101-3109 (1992-06-15)

SOCS3 silencing attenuates eosinophil functions in asthma patients.

Ma Paz Zafra et al.

International journal of molecular sciences, 16(3), 5434-5451 (2015-03-13)

Eosinophils are one of the key inflammatory cells in asthma. Eosinophils can exert a wide variety of actions through expression and secretion of multiple molecules. Previously, we have demonstrated that eosinophils purified from peripheral blood from asthma patients express high

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