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SRP3096

Sigma-Aldrich

Interleukin-6 human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

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Synonym(s):
Interleukin-6 human, IL-6
MDL number:
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥95% (SDS-PAGE)

form

lyophilized

potency

≤2.0 ng/mL ED50

mol wt

20.9 kDa

packaging

pkg of 100 μg
pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

-10 to -25°C

Gene Information

human ... IL6(3569)

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This Item
H7416SCU0001I1395
Sigma-Aldrich

Sigma-Aldrich

SRP3096

Interleukin-6 human

Interleukin-6 human IL-6, recombinant, expressed in HEK 293 cells, suitable for cell culture

Sigma-Aldrich

H7416

Interleukin-6 human

Interleukin-6 human recombinant, expressed in HEK 293 cells, suitable for cell culture

Sigma-Aldrich

SCU0001

Interleukin-6 human

Interleukin-6 human IL-6, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

I1395

Interleukin-6 human

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

form

lyophilized

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

20.9 kDa

mol wt

dimer 26-30 kDa (glycosylated)

mol wt

calculated mol wt 20.8 kDa (183 amino acids glycosylated protein)

mol wt

26 kDa

packaging

pkg of 100 μg, pkg of 20 μg

packaging

pkg of 10 μg

packaging

vial of ≥10 μg

packaging

pkg of 10 and 50 μg

General description

IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130 (membrane glycoprotein 130). The gene IL-6 is mapped to human chromosome 7p15.3. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kDa protein containing 184 amino acid residues.

Application

IL-6 human has been used for STAT3 (signal transducer and activator of transcription 3) activation. It has also been used as a standard for detection of IL-6 in Ben-Men-I cells (human WHO grade I meningioma derived, hTERT immortalized cells).

Biochem/physiol Actions

IL-6 (interleukin 6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease, and plasmacytoma/multiple myeloma.
Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Packaging

100 μg
20 μg

Sequence

PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

Physical form

Lyophilized from a 0.2 um filtered solution of phosphate buffered saline (PBS), pH 7.4, and stabilizers.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute the contents using endotoxin free water to a concentration of 100 ug/mL. For lower concentrations, the product should be diluted before use in medium containing at least 0.5% protein (i.e., cell culture medium containing 5–10% serum).

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

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