SRP3096

Interleukin-6 human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

• Synonym(s): Interleukin-6 human, IL-6
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00131403

Properties

• biological source: human
• Quality Level: 300
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• potency: ≤2.0 ng/mL ED₅₀
• mol wt: 20.9 kDa
• packaging: pkg of 100 μg, pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg endotoxin, tested
• UniProt accession no.: P05231
• shipped in: wet ice
• storage temp.: -10 to -25°C
• Gene Information: human ... IL6(3569)

Description

General description

IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130 (membrane glycoprotein 130). The gene IL-6 is mapped to human chromosome 7p15.3. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kDa protein containing 184 amino acid residues.

Application

IL-6 human has been used for STAT3 (signal transducer and activator of transcription 3) activation. It has also been used as a standard for detection of IL-6 in Ben-Men-I cells (human WHO grade I meningioma derived, hTERT immortalized cells).[1]

Biochem/physiol Actions

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β₂), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

IL-6 (interleukin 6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease, and plasmacytoma/multiple myeloma.

Packaging

100 μg

20 μg

Physical form

Lyophilized from a 0.2 um filtered solution of phosphate buffered saline (PBS), pH 7.4, and stabilizers.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute the contents using endotoxin free water to a concentration of 100 ug/mL. For lower concentrations, the product should be diluted before use in medium containing at least 0.5% protein (i.e., cell culture medium containing 5–10% serum).

Other Notes

PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319,H335
• pcodes: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable