After reconstitution with water. For extended storage, it is recommended to further dilute the solution in a buffer containing a carrier protein (example 0.1% BSA) and store it in working aliquots at -20°C to -80°C. As BSA is a significant component of FBS this would meet the long-term storage recommendation criteria.
SRP3098
IL-8 (77aa) (CXCL8) human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
CXCL8, NAP-1, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF)
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biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
mol wt
8.9 kDa
packaging
pkg of 25 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IL8(3576)
1 of 4
This Item | SRP3311 | SRP3099 | SRP3085 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source human | biological source human | biological source human | biological source human |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay ≥95% (HPLC) |
| form lyophilized | form lyophilized | form lyophilized | form (Lyophilized with no additives from a 0.2µ filtered solution) |
| packaging pkg of 25 μg | packaging pkg of 25 μg | packaging pkg of 10 μg | packaging pkg of 50 μg |
General description
Interleukin-8 (IL-8) is a proinflammatory CXC chemokine that can signal through the C-X-C motif chemokine receptors 1 and 2 (CXCR1 and 2). It is also called as C-X-C motif chemokine ligand 8 (CXCL8).[1][2] IL-8 is secreted by monocytes and endothelial cells. The gene encoding IL-8 is localized on human chromosome 4q13.3.[3] Recombinant human IL-8 (endothelial-derived) is an 8.9kDa protein containing 77 amino acid residues.
Application
IL-8 (77aa) (CXCL8) human has been used as a G protein-coupled receptor (GPCR) agonist.[4]
Biochem/physiol Actions
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Malefyt, R.
Cytokine Reference, 1061-1067 (2001)
K Matsushima et al.
The Journal of experimental medicine, 167(6), 1883-1893 (1988-06-01)
The cDNA coding for human monocyte-derived neutrophil-specific chemotactic factor (MDNCF) was cloned from LPS-stimulated human monocyte mRNA. The cDNA sequence codes for a polypeptide consisting of 99 amino acids, including a putative signal sequence. Comparison of the deduced amino acid
Nathan D Camp et al.
Pharmacological research, 105, 13-21 (2016-01-17)
G protein-coupled receptors (GPCRs) are essential membrane proteins that facilitate cell-to-cell communication and co-ordinate physiological processes. At least 30 human GPCRs contain a Type I PSD-95/DLG/Zo-1 (PDZ) ligand in their distal C-terminal domain; this four amino acid motif of X-[S/T]-X-[φ]
The chemokine and chemokine receptor superfamilies and their molecular evolution.
Zlotnik A
Genome Biology, 7(12), 243-243 (2006)
Prem Raj B Joseph et al.
The Biochemical journal, 472(1), 121-133 (2015-09-16)
Chemokine CXCL8/interleukin-8 (IL-8) plays a crucial role in directing neutrophils and oligodendrocytes to combat infection/injury and tumour cells in metastasis development. CXCL8 exists as monomers and dimers and interaction of both forms with glycosaminoglycans (GAGs) mediate these diverse cellular processes.
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AFTER THE INITIAL RECONSTITUTION IN WATER, CAN THIS BE DILUTED IN PBS WITH 10% FBS?
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