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SRP3114

Sigma-Aldrich

Midkine human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

MK, NEGF-2

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20 μG
$356.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

$356.00


Usually ships in 2 weeks.

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

13.4 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MDK(4192)

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This Item
AG243SRP3010SRP3183
recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli

recombinant

expressed in E. coli

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>98% (SDS-PAGE and HPLC)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

biological source

human

biological source

human

biological source

human

biological source

human

form

lyophilized

form

powder

form

lyophilized

form

lyophilized

UniProt accession no.

P21741

UniProt accession no.

P21741

UniProt accession no.

Q02223

UniProt accession no.

P49765

General description

Recombinant human Midkine is a 13.4 kDa protein containing 123 amino acid residues including five intra-molecular disulfide bonds.

Application

Midkine human has been used in cell viability assay and cell proliferation assay to determine its association with EMT (epithelial-mesenchymal transition) and gemcitabine resistance in biliary tract cancer.[1]

Biochem/physiol Actions

Midkine (MK) and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK).[2] MK (midkine) plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development.[3] MK chemoattracts embryonic neurons, neutrophils and macrophages.[3][4] Signaling through the ALK (anaplastic lymphoma kinase) receptor MK exerts angiogenic, growth and survival activities during tumorigenesis.[5] MK is expressed predominantly during midgestation in the brain and several other organs and is down-regulated at birth.[5]

Sequence

VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD

Physical form

Lyophilized from 0.4x PBS, pH 7.4.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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