SRP3124

Neurturin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): NRTN, NTN
• NACRES: NA.75
• UNSPSC Code: 12352202

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC), ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: 11.8 kDa
• packaging: pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: Q99748
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... NRTN(4902)

Description

General description

Neurturin (NTN) is a disulfide-linked homodimer neurotrophic factor structurally related to glial cell derived neurotrophic factor (GDNF), artemin, and persephin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures.[1][2] The functional form of human Neurturin is a disulfide-linked homodimer, of two 11.8kDa polypeptide monomers (206 total amino acid residues). Each monomer contains seven conserved cysteine residues, one of which (Cys 69) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Biochem/physiol Actions

Neurturin (NTN) signals through a multicomponent receptor system, composed of RET and one of four GFRα (α1-α4) receptors. Neurturin promotes the development and survival of sympathetic and sensory neurons by signaling through a receptor system composed of RET and GFRα2. NTN modulates spermatogenesis, kidney morphogenesis and controls smooth muscle of penile blood vessels and several intrapelvic organs.[3][4]

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 4.0.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

MARLGARPCG LRELEVRVSE LGLGYASDET VLFRYCAGAC EAAARVYDLG LRRLRQRRRL RRERVRAQPC CRPTAYEDEV SFLDAHSRYH TVHELSAREC ACV

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable