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SRP3147

Sigma-Aldrich

Relaxin-2 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):
H2 relaxin, RLN2
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

6.0 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... RLN2(6019)

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Sigma-Aldrich

Sigma-Aldrich

SRP3147

Relaxin-2 human

Sigma-Aldrich

Sigma-Aldrich

R2156

Relaxin-1 human

Sigma-Aldrich

Sigma-Aldrich

SRP6233

Relaxin-3 human

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

-

assay

>95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

form

lyophilized

form

buffered aqueous solution

form

lyophilized powder

form

lyophilized powder

mol wt

6.0 kDa

mol wt

21043 Da

mol wt

(Contains two disulfide-linked subunits: A chain ~2.8 kDa B chain ~3.3 kDa)

mol wt

5.5 kDa

packaging

pkg of 25 μg

packaging

-

packaging

-

packaging

pkg of 10 μg, pkg of 50 μg

General description

Relaxin-2 is a peptide hormone structurally related to insulin. The gene is mapped to human chromosome 9p24. It is expressed in the placenta, decidua, prostate, corpus luteum, endometrium, mammary glands, heart, and brain. It is also expressed in the ovary during pregnancy. Recombinant relaxin-2 is a nonglycosylated 6kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 29 amino acid B-chain.

Application

It has also been used to study its effect on human endometrial carcinoma cell lines.
Relaxin-2 human has been used to study the effect of testosterone on the expression of relaxin receptor in the knee joint.

Biochem/physiol Actions

Of the three known relaxin genes, Relaxin-2 (RLN2) is the only relaxin known to circulate in the blood. Relaxin-2 binds specifically to the LGR7 (leucine-rich repeat-containing G-protein coupled receptor 7) and LGR8 receptors, previously identified as an "orphan" G protein coupled receptors. Signaling by Relaxin-2 through its target receptors enhances the growth of pubic ligaments and ripening of the cervix during birth. Relaxin-2 also participates in cancer progression. In prostate cancer, it is responsible for tumor growth and vascularisation. Relaxin-2 is also linked with anti-fibrotic and cardioprotective effects.

Sequence

A-Chain: QLYSALANKC CHVGCTKRSL ARFC B-Chain: DSWMEEVIKL CGRELVRAQI AICGMSTWS

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Human relaxin-2: historical perspectives and role in cancer biology.
Nair VB, et al.
Amino Acids, 43, 1131-1140 (2012)
Dual blockade of PKA and NF-?B inhibits H2 relaxin-mediated castrate-resistant growth of prostate cancer sublines and induces apoptosis.
Vinall RL, et al.
Hormones & Cancer, 2, 224-238 (2011)
Relaxin 2/RXFP1 Signaling Induces Cell Invasion via the beta-Catenin Pathway in Endometrial Cancer
Fue M, et al.
International Journal of Molecular Sciences, 19(8), 2438-2438 (2018)
Relaxin as a cardiovascular hormone: physiology, pathophysiology and therapeutic promises.
Nistri S, et al.
Cardiovascular & Hematological Agents in Medicinal Chemistry, 5, 101-108 (2007)
Analysis of the 5'-upstream regions of the human relaxin H1 and H2 genes and their chromosomal localization on chromosome 9p24.1 by radiation hybrid and breakpoint mapping.
Garibay-Tupas JL, et al.
Journal of Molecular Endocrinology, 23, 355-365 (1999)

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