SRP3150

Sigma-Aldrich

sCD23 human

recombinant, expressed in E. coli, ≥96% (SDS-PAGE), ≥96% (HPLC), suitable for cell culture

Synonym(s):
Fc-epsilon-RII, BLAST-2, CD23 antigen, Lymphocyte IgE receptor
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in E. coli

assay

≥96% (HPLC)
≥96% (SDS-PAGE)

form

lyophilized

mol wt

19.2 kDa

packaging

pkg of 20 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FCER2(2208)

General description

CD23, the low affinity receptor for IgE, belongs to the C-type lectin structural family and plays a role in the regulation of IgE synthesis and IgE mediated activities. It is found both as a transmembrane receptor protein and in a soluble form, which is generated by proteolytic cleavage of membrane bound CD23. The predominant soluble form of CD23 (sCD23) consists of 172 amino acids corresponding to the extracellular domain of the full length precursor. sCD23, in addition to binding IgE, also exerts a number of IgE independent activities, such as promoting the activation and differentiation of B-cells and stimulating the release of pro-inflammatory cytokines from monocytes. Recombinant human sCD23 is a 19.2 kDa non-glycosylated protein containing 172 amino-acid residues.

Biochem/physiol Actions

CD23, the low affinity receptor for IgE, belongs to the C-type lectin structural family and plays a role in the regulation of IgE synthesis and IgE mediated activities. Recombinant human sCD23 is a 19.2 kDa non-glycosylated protein containing 172 amino-acid residues.

Sequence

MELQVSSGFV CNTCPEKWIN FQRKCYYFGK GTKQWVHARY ACDDMEGQLV SIHSPEEQDF LTKHASHTGS WIGLRNLDLK GEFIWVDGSH VDYSNWAPGE PTSRSQGEDC VMMRGSGRWN DAFCDRKLGA WVCDRLATCT PPASEGSAES MGPDSRPDPD GRLPTPSAPL HS

Physical form

Lyophilized from 0.5x PBS, pH 8.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
G Delespesse et al.
International archives of allergy and applied immunology, 90 Suppl 1, 41-44 (1989-01-01)
The low-affinity receptor for IgE (FcERII or CD23) is a membrane 45-kD glycoprotein which is cleaved by an autoproteolytic mechanism into soluble 37-, 33- and 25-kD fragments that are capable of binding to IgE (IgE-binding factors, IgE-BFs). FcERIIa (which is...
A Saxon et al.
The Journal of allergy and clinical immunology, 86(3 Pt 1), 333-344 (1990-09-01)
The role of soluble fragments of CD23 and their relationship to interleukin-4 (IL-4) in the in vitro production of IgE by normal human peripheral blood mononuclear cells was examined. Most donors' cells were induced to produce IgE in vitro by...

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