MilliporeSigma
All Photos(1)

Documents

SRP3171

Sigma-Aldrich

TGF-beta 3-E. coli human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Transforming Growth Factor-beta3
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.05 ng/mL ED50

mol wt

25.0 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TGFB3(7043)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
H8791T5425GF317
vibrant-m

SRP3171

TGF-beta 3-E. coli human

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

>98% (SDS-PAGE and HPLC)

biological source

human

biological source

human

biological source

human

biological source

-

packaging

pkg of 10 μg

packaging

pkg of 5 μg

packaging

pkg of 2 μg

packaging

-

mol wt

25.0 kDa

mol wt

dimer 25 kDa (non-glycosylated)

mol wt

predicted mol wt ~25 kDa

mol wt

-

General description

The gene TGF-β3 (transforming growth factor β 3) is mapped to human chromosome 14q24.3. The three mammalian isoforms of TGF-β, TGF-β1, β2, β3 elicit similar biological responses. TGF-β proteins are made as precursor molecules which are cut to generate a mature carboxy-terminal region of 110-140 amino acids. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. Recombinant TGF-β3 is a 25.0kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

Application

TGF-β3 (transforming growth factor β 3) human has been used in the media for the chondrogenic differentiation of the stromal cells. It has been used in the culture medium to allow attachment of primary human dermal fibroblasts to the scaffolds.

Biochem/physiol Actions

The three mammalian isoforms of TGF-β (transforming growth factor β), TGF-β1, β2, β3, are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. TGF-β3 is the predominant TGF-β in the testis and controls the blood-testis barrier activity. Polymorphisms in this gene might be associated with male infertility. Heterozygous mutation in the gene at position Arg300 might be associated with increased TGF-β signaling, leading to a disorder with phenotypic overlap with Marfan syndrome and Loeys-Dietz syndrome. TGF-β3 is involved in the mesenchymal stem cells (MSCs)-mediated inhibition of fibroblast proliferation and decreases skin fibrosis.

Sequence

ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS

Physical form

Lyophilized from 0.1% TFA

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 °C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 °C to -80 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media.
Amable PR
PLoS ONE, 9, e104662-e104662 (2014)
Femur bone marrow from brain death deceased donors as source of human mesenchymal stromal cells for cell therapy.
Echarte, et al.
Stem cell investigation, 10, 12-12 (2023)
Identification of appropriate reference genes for human mesenchymal cells during expansion and differentiation.
Amable PR
PLoS ONE, 8, e73792-e73792 (2013)
TGF?3 (TGFB3) polymorphism is associated with male infertility.
Drozdzik M
Scientific Reports, 5 (2015)
Association between TGFB3 and nonsyndromic cleft lip with or without cleft palate in a Chilean population.
Suazo J
The Cleft Palate-Craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association, 47, 513-517 (2010)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service