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SRP3177

Sigma-Aldrich

TNF-α human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

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Synonym(s):
Cachectin, Cytotoxin, Differentiation-inducing factor (DIF), Necrosin, Tumor Necrosis Factor
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

lyophilized

potency

≤0.05 ng/mL ED50

mol wt

17.4 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg endotoxin

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TNF(7124)

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This Item
H891611088939001SRP3162
vibrant-m

SRP3177

TNF-α human

vibrant-m

H8916

Tumor Necrosis Factor-α human

vibrant-m

SRP3162

sTNF-receptor Type I human

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in yeast

recombinant

expressed in E. coli

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

-

form

lyophilized

form

lyophilized powder

form

solution

form

lyophilized

General description

TNF-α (tumor necrosis factor α) is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. The gene is mapped to human chromosome 6p21.3. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Application

TNF-α (tumor necrosis factor α) human has been used as a positive control for induction of proinflammatory genes in human intestinal and hepatic cell lines.

Biochem/physiol Actions

TNF-α (tumor necrosis factor α) is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. However, it can be a tumor promoter as well as a tumor suppressor. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, apoptosis and diabetes. It is also associated with mood and anxiety disorders.

Sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 0.5mM DTT.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Regulation of cytokines, cytokine inhibitors, and acute-phase proteins following anti-TNF-alpha therapy in rheumatoid arthritis.
Charles P
Journal of Immunology, 163, 1521-1528 (1999)
Breast cancer cells induce stromal fibroblasts to express MMP-9 via secretion of TNF-alpha and TGF-beta.
Stuelten CH
Journal of Cell Science, 118, 2143-2153 (2005)
Immunogenicity of Anti-TNF-alpha agents in autoimmune diseases.
Emi Aikawa N
Clinical Reviews in Allergy & Immunology, 38, 82-89 (2010)
Depressive symptoms are associated with tumor necrosis factor alpha in systemic lupus erythematosus.
Postal M
Journal of Neuroinflammation, 13, 5-5 (2016)
Cachetin/TNF-alpha in septic shock and septic adult respiratory distress syndrome.
Tracey KJ
The American Review of Respiratory Disease, 138, 1377-1379 (1988)

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