SRP3190

Sigma-Aldrich

Betacellulin from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
BTC
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.01 ng/mL ED50

mol wt

9.0 kDa

packaging

pkg of 20 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... BTC(12223)

General description

Betacellulin is a member of the EGF family of growth factors and is produced by an expression of a DNA sequence encoding the mature 80 amino acid residue polypeptide. The protein is expressed in the small intestine and pancreas. It is initially synthesized as a glycosylated 32.0kDa transmembrane precursor protein, processed by proteolytic cleavage to produce the mature sequence.

Application

This product has been used to study differentiation of pancreatic acinar cell line (AR42J) into insulin-secreting cells and has been linked to a role in the growth and differentiation of islet cells in human pancreas.

Biochem/physiol Actions

Betacellulin and the other members of the epidermal growth factor (EGF) family of growth factors bind with high affinity to both EGFR and to erb-b2 receptor tyrosine kinase 4 (erbB-4). Overexpression of betacellulin with erbB-3 and erbB-4 has been linked to human endometrial adenocarcinoma.

Caution

This product should be stored at -2-8°C. For longer-term storage, it is recommended to dilute in a buffer containing a carrier protein, for example, 0.1% BSA, and to store the solution frozen in working aliquots at 20°C or -80°C.

Sequence

DGNTTRTPET NGSLCGAPGE NCTGTTPRQK VKTHFSRCPK QYKHYCIHGR CRFVVDEQTP SCICEKGYFG ARCERVDLFY

Preparation Note

This product was lyophilized with no additives. It should be reconstituted by adding to water at a concentration of 0.1-1.0 mg/mL. The vial should be centrifuged prior to opening and the solution should not be vortexed.

Analysis Note

The biological activity of this product is measured by an ability to stimulate H-thmidine incorporation in EGF-responsive mouse BALB/c 3T3 fibroblasts. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Expression of the c-erbB-3/HER-3 and c-erbB-4/HER-4 growth factor receptors and their ligands, neuregulin-1 alpha, neuregulin-1 beta, and betacellulin, in normal endometrium and endometrial cancer.
Srinivasan R
Clinical Cancer Research (1999)
Human betacellulin, a member of the EGF family dominantly expressed in pancreas and small intestine, is fully active in a monomeric form.
Growth Factors (1996)
Different Epidermal Growth Factor (EGF) Receptor Ligands Show Distinct Kinetics and Biased or Partial Agonism for Homodimer and Heterodimer Formation*
Jennifer L
The Journal of Biological Chemistry (2014)
M Seno et al.
Growth factors (Chur, Switzerland), 13(3-4), 181-191 (1996-01-01)
Betacellulin (BTC) was found to be expressed mainly in human pancreas and small intestine. This finding suggests that BTC possesses some specific function distinguished from the other members of epidermal growth factor (EGF) family. To clarify this function, the released...

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