SRP3197

Sigma-Aldrich

FGF-acidic from mouse

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

Synonym(s):
HBGF-1, FGF-1, Fibroblast Growth Factor-acidic, ECGF-beta
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

mouse

recombinant

expressed in E. coli

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

≤0.5 ng/mL

mol wt

15.9 kDa

packaging

pkg of 50 μg

application(s)

cell culture | mammalian: suitable

impurities

0.0344 EU/μg endotoxin, tested

color

off-white to yellow

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... FGF1(14164)

General description

FGF1 (fibroblast growth factor 1) belongs to the FGF family of genes. In humans, FGF1 is alternatively spliced into four forms: FGF1A (in kidney), FGF1B (in brain), FGF1-C and -D (in vascular smooth muscle cells and fibroblasts). FGF1 interacts with tyrosine kinase FGF receptors (FGFR1-4) with high affinity. It can translocate between the cytosol and nucleus.
Recombinant murine FGF-acidic is a 15.9kDa protein consisting of 141 amino acid residues.

Application

FGF-acidic from mouse has been used to induce the expression of PTPIP51 (protein tyrosine phosphatase interacting protein 51) in mouse hippocampal cells.

Biochem/physiol Actions

FGF1 (fibroblast growth factor 1) is mainly involved in cell growth, proliferation and neurogenesis. In high-fat diet (HFD) mice, FGF1 is upreglated in adipose tissue. Absence of FGF1 in mice results in aggressive diabetic phenotype as well as adipose expansion in the presence of HFD. The PPARγ (peroxisome proliferator-activated receptor γ)-FGF1 axis helps in regulating metabolic homeostasis and insulin sensitization in mice. FGF1 also participates in wound healing, post-ischemic heart repair and making of collaterals after hindlimb ischemia.

Sequence

MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Physical form

Lyophilized from 5 mM Sodium Phosphate, pH 7.2 + 100 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 5mM Sodium Phosphate, pH 7.0-7.2 to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Torunn Sletten et al.
PloS one, 9(3), e90687-e90687 (2014-03-07)
Extracellular fibroblast growth factor 1 (FGF1) acts through cell surface tyrosine kinase receptors, but FGF1 can also act directly in the cell nucleus, as a result of nuclear import of endogenously produced, non-secreted FGF1 or by transport of extracellular FGF1...
Regulation of FGF1 gene promoter through transcription factor RFX1.
Hsu YC, et al.
The Journal of Biological Chemistry, 285, 13885-13885 (2010)
A PPAR?-FGF1 axis is required for adaptive adipose remodelling and metabolic homeostasis.
Jonker JW, et al.
Nature, 485, 391-391 (2012)
Aleksandr Kirov et al.
PloS one, 7(5), e36485-e36485 (2012-05-19)
FGF1 is a signal peptide-less nonclassically released growth factor that is involved in angiogenesis, tissue repair, inflammation, and carcinogenesis. The effects of nonclassical FGF export in vivo are not sufficiently studied. We produced transgenic mice expressing FGF1 in endothelial cells...
Fibroblast growth factors.
Ornitz, D.M., et al.
Genome Biology, 2, 30051-300512 (2001)

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