SRP3200

Sigma-Aldrich

GDNF from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
ATF-1, Glial-Derived Neurotrophic Factor
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.2 ng/mL

mol wt

15.1 kDa

packaging

pkg of 10 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... GDNF(14573)

General description

GDNF (glial cell line derived neurotrophic factor) is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. The functional murine GDNF ligand is a disulfide-linked homodimer, of two 15.1kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Application

GDNF from mouse has been used for stimulating primary murine cultures of dorsal root ganglion (DRG) neurons.
GDNF from mouse has been used to study the role of NEDL2 (Nedd4-related E3 ubiquitin ligase-2) in GDNF/Ret signaling.

Biochem/physiol Actions

GDNF (glial cell line derived neurotrophic factor) signals through a multicomponent receptor system, composed of a RET (receptor tyrosine kinase) and one of the four GFR-α (α1-α4) receptors (GDNF family receptors). GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson′s disease monkey model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. It might also be involved in the treatment of non-alcoholic fatty liver disease. GDNF is needed for proper spermatogonial stem cell survival and proliferation.

Sequence

MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Physical form

Lyophilized from 10 mM Sodium Citrate.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Peritubular myoid cells participate in male mouse spermatogonial stem cell maintenance.
Chen LY, et al.
Endocrinology, 155, 4964-4974 (2014)
GDNF - a stranger in the TGF-beta superfamily?
Saarma M
European Journal of Biochemistry, 267, 6968-6971 (2000)
NEDL2 is an essential regulator of enteric neural development and GDNF/Ret signaling.
Wei R, et al.
Cellular Signalling, 27, 578-586 (2015)
Characterization of a multicomponent receptor for GDNF.
Treanor JJ, et al.
Nature, 382, 80-83 (1996)
Peripheral expression and biological activities of GDNF, a new neurotrophic factor for avian and mammalian peripheral neurons.
Trupp M, et al.
The Journal of Cell Biology, 130, 137-148 (1995)

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