SRP3211

IL-4 from mouse

Name: IL-4 from mouse
Brand: SIGMA
Price: 337 USD

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

B cell stimulating factor (BSF-1), BCDF, BCGF

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20 μG

$337.00

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.32

Key Documents

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biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤2.0 ng/mL ED₅₀

mol wt

13.7 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

P07750

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... IL4(16189)

Related Categories

Animal Component Free Cell Culture Reagents

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Growth Factors and Cytokines

Molecular Biology & Functional Genomics

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Show Differences

1 of 4

This Item	SRP3093	SRP3204	SRP3212
Sigma-Aldrich SRP3211 IL-4 from mouse	Sigma-Aldrich SRP3093 IL-4 human	Sigma-Aldrich SRP3204 IL-12 from mouse	Sigma-Aldrich SRP3212 IL-5 from mouse
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
biological source mouse	biological source human	biological source mouse	biological source mouse
assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in CHO cells	recombinant expressed in E. coli
form lyophilized	form lyophilized	form lyophilized	form lyophilized
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

General description

IL-4 (interleukin 4) is a pleiotropic cytokine.[1] Produced by mast cells, T cells and bone marrow stromal cells.[2][3] Recombinant murine IL-4 is a 13.7kDa globular protein containing 120 amino acid residues.

Biochem/physiol Actions

It regulates diverse T and B cell responses including cell proliferation, survival and gene expression.[2] IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 (T helper) cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response.[2] Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 (immunoglobulin G1) and IgE isotypes.[4] Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.[5] IL-4 helps in long-term recovery after cerebral ischemia by controlling the activities of microglia/macrophage.[6] In mice, absence of IL-4 is associated with a state anxiety-like behavior.[7]

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Other Notes

MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Using Stable Isotopes in Bone Marrow Derived Macrophage to Analyze Metabolism.

Chih-Wei Ko et al.

Bio-protocol, 8(17), e3003-e3003 (2018-09-05)

Using gas chromatography mass spectrometry (GC-MS) to analyze the citric acid cycle (CAC) and related intermediates (such as glutamate, glutamine, GABA, and aspartate) is an analytical approach to identify unexpected correlations between apparently related and unrelated pathways of energy metabolism.

Mast cell production and response to IL-4 and IL-13.

McLeod JJ

Cytokine, 75, 57-61 (2015)

Interleukin-4 Is Essential for Microglia/Macrophage M2 Polarization and Long-Term Recovery After Cerebral Ischemia.

Liu X

Stroke, 47, 498-504 (2016)

Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4.

Hasbold J

European Journal of Immunology, 28, 1040-1051 (1998)

IL-17A as an inducer for Th2 immune responses in murine atopic dermatitis models.

Saeko Nakajima et al.

The Journal of investigative dermatology, 134(8), 2122-2130 (2014-02-01)

Atopic dermatitis (AD) is generally regarded as a type 2 helper T (Th2)-mediated inflammatory skin disease. Although the number of IL-17A-producing cells is increased in the peripheral blood and in acute skin lesion of AD patients, the role of IL-17A

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