SRP3228

Sigma-Aldrich

PDGF-AA from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
Osteosarcoma-derived Growth Factor (ODGF), Glioma-derived growth factor (GDGF)
eCl@ss:
32190102
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

8-10 ng/mL ED50

mol wt

28.7 kDa

packaging

pkg of 10 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... PDGFA(18590)

General description

The PDGFs are stored in platelet α-granules and are released upon platelet activation. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids). Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids).

Application

PDGF-AA from mouse has been used as a supplement in the media used for differentiation of neurospheres.

Biochem/physiol Actions

The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-AA is crucial for optimal wound healing.

Sequence

MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Native and oxidized LDL enhances production of PDGF AA and the surface expression of PDGF receptors in cultured human smooth muscle cells.
Stiko-Rahm A
Arteriosclerosis and Thrombosis, 12, 1099-1109 (1992)
Interleukin-1 mitogenic activity for fibroblasts and smooth muscle cells is due to PDGF-AA.
Raines EW
Science, 243, 393-396 (1989)
Isolation of neural stem cells from the postnatal cerebellum.
Lee A
Nature Neuroscience, 8, 723-729 (2005)
An essential role for senescent cells in optimal wound healing through secretion of PDGF-AA.
Demaria M
Developmental Cell, 31, 722-733 (2014)
Detection of platelet-derived growth factor (PDGF)-AA in actively healing human wounds treated with recombinant PDGF-BB and absence of PDGF in chronic nonhealing wounds.
Pierce GF
The Journal of Clinical Investigation, 96, 1336-1350 (1995)

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