SRP3230

Sigma-Aldrich

Persephin from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
PSP, PSPN
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.1 ng/mL ED50

mol wt

10.3 kDa

packaging

pkg of 20 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... PSPN(19197)

General description

Persephin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF (glial cell line-derived neurotrophic factor), Artemin, and Neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFRα (α1-α4) receptors. The GFRα4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. Persephin promotes the survival of ventral midbrain dopaminergic neurons and motor neurons after sciatic nerve axotomy, and like GNDF, promotes ureteric bud branching. Recombinant murine Persephin is a disulfide-linked homodimer, composed of two 10.3 kDa polypeptide chains (96 total amino acid residues). Each chain contains seven conserved cysteine residues, one of which (Cys 63) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. Persephin is one of the GDNF family ligands and belongs to transforming growth factor β (TGF-β) superfamily.

Biochem/physiol Actions

In contrast to GDNF (glial cell line-derived neurotrophic factor) and neurturin, persephin does not support survival of peripheral neurons. Persephin induces differentiation of mesencephalic dopaminergic neurons.

Sequence

ALAGSCRLWS LTLPVAELGL GYASEEKVIF RYCAGSCPQE ARTQHSLVLA RLRGRGRAHG RPCCQPTSYA DVTFLDDQHH WQQLPQLSAA ACGCGG

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 4.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Transforming growth factor β cooperates with persephin for dopaminergic phenotype induction.
Roussa E, et al.
Stem Cells, 26(7), 1683-1694 (2008)
The RET receptor: function in development and dysfunction in congenital malformation.
Manie S, et al.
Trends in Genetics, 17(10), 580-589 (2001)
Characterization of a multicomponent receptor for GDNF.
Treanor J J, et al.
Nature, 382(6586), 80-80 (1996)
M Saarma
Trends in neurosciences, 24(8), 427-429 (2001-07-31)
Glial cell line-derived neurotrophic factor (GDNF) family ligands (GFL) are potent survival factors and regulators for central and peripheral neurons. GFLs bind to specific glycosyl phosphatidylinositol (GPI)-anchored co-receptors (GFRalpha1-alpha4), but signal through a common c-Ret receptor. Both GPI-anchored and soluble...
M Lindahl et al.
Molecular and cellular neurosciences, 15(6), 522-533 (2000-06-22)
Members of the GDNF protein family signal through receptors consisting of a GPI-linked GFRalpha subunit and the transmembrane tyrosine kinase Ret. Here we characterize the mouse Gfra4 and show that it undergoes developmentally regulated alternative splicing in several tissues. The...

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