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SRP3234

Sigma-Aldrich

SCF from mouse

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):
Mast Cell Growth Factor (MGF), Steel Factor, Stem Cell Factor, c-Kit Ligand
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤10.0 ng/mL ED50

mol wt

18.3 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... KITL(17311)

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GF141S9915GF021
Sigma-Aldrich

Sigma-Aldrich

SRP3234

SCF from mouse

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>98% (SDS-PAGE)

assay

≥95% (SDS-PAGE and HPLC)

assay

>98% (SDS-PAGE and HPLC)

form

lyophilized

form

-

form

powder

form

-

mol wt

18.3 kDa

mol wt

-

mol wt

18.3 kDa

mol wt

-

packaging

pkg of 10 μg

packaging

-

packaging

pkg of 10 μg, pkg of 50 μg

packaging

-

General description

Stem cell factor (SCF), which is also known as kit ligand (KITL), is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant murine SCF is an 18.3kDa polypeptide containing 164 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

Biochem/physiol Actions

SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. In mice, this protein has been shown to improve the development of ovarian follicles.

Sequence

MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 6.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Edyta E Wojtowicz et al.
Experimental hematology, 42(10), 909-918 (2014-08-06)
MicroRNAs (miRNAs) are crucial for proper functioning of hematopoietic stem and progenitor cells (HSPCs). Members of the miRNA-125 family (consisting of miR-125a, miR-125b1, and miR-125b2) are known to confer a proliferative advantage on cells upon overexpression, to decrease the rate
Laura Pitzonka et al.
PloS one, 9(5), e97628-e97628 (2014-05-17)
Co-transcriptionally assembled ribonucleoprotein (RNP) complexes are critical for RNA processing and nuclear export. RNPs have been hypothesized to contribute to the regulation of coordinated gene expression, and defects in RNP biogenesis contribute to genome instability and disease. Despite the large
Kit ligand decreases the incidence of apoptosis in cultured vitrified whole mouse ovaries.
Abdi S
Reproductive Biomedicine Online, 30(5), 493-503 (2015)
D M Anderson et al.
Cell, 63(1), 235-243 (1990-10-05)
We have previously reported the identification of a novel mast cell growth factor (MGF) that was shown to be a ligand for c-kit and is encoded by a gene that maps near the steel locus on mouse chromosome 10. We
T R Ulich et al.
Blood, 78(3), 645-650 (1991-08-01)
Recombinant rat stem cell factor (rrSCF) administered to rats as a single intravenous injection causes a dose-dependent neutrophilia and lymphocytosis as well as the appearance of immature myeloid cells and occasional blast cells in the circulation. Neutrophilia begins at 2

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