SRP3247

Sigma-Aldrich

M-CSF from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
MGI-IM, CSF-1, Macrophage Colony Stimulating Factor
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

rat

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤5.0 ng/mL ED50

mol wt

18.1 kDa

packaging

pkg of 10 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.2 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... CSF1(78965)

General description

Macrophage colony stimulating factor (M-CSF) is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts.The gene encoding this protein is localized on rat chromosome 2q34. The human protein is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant rat M-CSF is a 36.2kDa homodimeric protein containing two 155 amino acid polypeptide subunits.

Biochem/physiol Actions

Macrophage colony stimulating factor (M-CSF) is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation.

Sequence

MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP

Physical form

Lyophilized from 5 mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
The osteopetrotic mutation toothless (tl) is a loss-of-function frameshift mutation in the rat Csf1 gene: Evidence of a crucial role for CSF-1 in osteoclastogenesis and endochondral ossification.
Van Wesenbeeck L
Proceedings of the National Academy of Sciences of the USA, 99(22), 14303-14308 (2002)
Circulating colony stimulating factor-1 and breast cancer risk.
Tamimi RM
Cancer Research, 68(1), 18-21 (2008)
Stanley, E.R., et al.
The Cytokine Handbook, 387-418 (1994)
Jonah B Sacha et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(24), 9791-9796 (2009-05-30)
The precise immunological role played by CD4(+) T cells in retroviral infections is poorly defined. Here, we describe a new function of these cells, the elimination of retrovirus-infected macrophages. After experimental CD8(+) cell depletion, elite controlling macaques with set-point viral...
Colony-stimulating factor-1 antibody reverses chemoresistance in human MCF-7 breast cancer xenografts.
Paulus P
Cancer Research, 66(8), 4349-4356 (2006)

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