SRP3292

R-SPONDIN-1 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE)

• Synonym(s): Rspo1
• NACRES: NA.32
• UNSPSC Code: 12352202

Properties

• biological source: human
• recombinant: expressed in CHO cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• potency: ≤50 ng/mL ED₅₀
• mol wt: monomer (26.8 kDa (243 amino acids))
• packaging: pkg of 20 μg
• impurities: <0.1 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: Q2MKA7
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... RSPO1(284654)

Description

General description

R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators.[1] Currently, the (Rspo) family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in adrenal glands, ovary, testis, thyroid, and trachea. Recombinant human R-Spondin-1 is a 26.7kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0kDa by SDS PAGE analysis under reducing conditions.

Biochem/physiol Actions

Rspo (R-Spondin) can interact with the Frizzled/LRP6 (LDL receptor related protein 6) receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Disruption of Rspo-1 gene causes recessive syndrome with XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Rspo-1 is also associated with radioresistance of tumor cells.[2]

Physical form

Lyophilized from a 0.2 μm filtered solution containingPhosphate Buffered Saline (PBS)

Preparation Note

Sterile water at 0.1 mg/mLCentrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable