SRP3309

GDNF human

Name: GDNF human
Brand: SIGMA
Price: 491 USD

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

ATF-1

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10 μG

$491.00

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

Key Documents

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biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.1 μg/mL

mol wt

30.0 kDa

packaging

pkg of 10 μg

impurities

endotoxin, tested

UniProt accession no.

P39905

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GDNF(2668)

Related Categories

Animal Component Free Cell Culture Reagents

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Growth Factors and Cytokines

Molecular Biology & Functional Genomics

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Show Differences

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This Item	SRP3200	SRP3239	SRP3014
Sigma-Aldrich SRP3309 GDNF human	Sigma-Aldrich SRP3200 GDNF from mouse	Sigma-Aldrich SRP3239 GDNF from rat	Sigma-Aldrich SRP3014 BDNF human
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
biological source human	biological source mouse	biological source rat	biological source human
assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥95% (SDS-PAGE)
form lyophilized	form lyophilized	form lyophilized	form lyophilized
UniProt accession no. P39905	UniProt accession no. P48540	UniProt accession no. Q07731	UniProt accession no. P23560
impurities endotoxin, tested	impurities <0.1 EU/μg endotoxin, tested	impurities <0.1 EU/μg endotoxin, tested	impurities ≤1.00 EU/μg endotoxin, tested

General description

Glial cell derived neurotrophic factor (GDNF) is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. The gene encoding this protein is localized on human chromosome 5p13.2. The functional human GDNF ligand is a disulfide-linked homodimer, of two 15kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Application

Glial cell derived neurotrophic factor (GDNF) has been used as a supplement in dorsal root ganglion (DRG) culture medium to study the effect of growth factors on neuron viability, neurite outgrowth and arborisation. (3)

Biochem/physiol Actions

Glial cell derived neurotrophic factor (GDNF) signals through a multicomponent receptor system, composed of a RET and one of the four GFR-α (α1-4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson′s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.

Physical form

Lyophilized from 10 mM Sodium Citrate + 50 mM NaCl.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

1112AFC64

0107S243

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Enrique Pimentel

Handbook of Growth Factors, Volume 2 null

Analysis of RET, ZEB2, EDN3 and GDNF genomic rearrangements in 80 patients with Hirschsprung disease (using multiplex ligation-dependent probe amplification).

Serra A

Annals of Human Genetics, 73(2), 147-151 (2009)

Primary Postnatal Dorsal Root Ganglion Culture from Conventionally Slaughtered Calves.

Fadda A

PLoS ONE, 11(12), e0168228-e0168228 (2016)

Controlled release and gradient formation of human glial-cell derived neurotrophic factor from heparinated poly(ethylene glycol) microsphere-based scaffolds.

Jacob L Roam et al.

Biomaterials, 35(24), 6473-6481 (2014-05-13)

Introduction of spatial patterning of proteins, while retaining activity and releasability, is critical for the field of regenerative medicine. Reversible binding to heparin, which many biological molecules exhibit, is one potential pathway to achieve this goal. We have covalently bound

GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons.

L F Lin et al.

Science (New York, N.Y.), 260(5111), 1130-1132 (1993-05-21)

A potent neurotrophic factor that enhances survival of midbrain dopaminergic neurons was purified and cloned. Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. In embryonic

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