SRP3316

LIF human

Name: LIF human
Brand: SIGMA
Price: 483 USD

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

D factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor (MLPLI)

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25 μG

$483.00

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

Key Documents

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recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

19.6 kDa

impurities

endotoxin, tested

UniProt accession no.

P15018

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... LIF(3976)

Related Categories

Animal Component Free Cell Culture Reagents

Cell Culture & Analysis

Molecular Biology & Functional Genomics

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This Item	LIF1050	LIF1005	SRP3321
Sigma-Aldrich SRP3316 LIF human	Sigma-Aldrich LIF1050 Leukemia Inhibitory Factor human	Sigma-Aldrich LIF1005 Leukemia Inhibitory Factor human	Sigma-Aldrich SRP3321 MIF human
recombinant expressed in E. coli	recombinant -	recombinant -	recombinant expressed in insect cells
assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay >95% (SDS-PAGE)	assay >95% (HPLC and SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)
form lyophilized	form liquid	form liquid	form lyophilized
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
Gene Information human ... LIF(3976)	Gene Information human ... LIF(3976)	Gene Information human ... LIF(3976)	Gene Information human ... MIF(4282)
UniProt accession no. P15018	UniProt accession no. P15018	UniProt accession no. P15018	UniProt accession no. P14174

General description

Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines.[1][2] This protein was originally identified as a protein that prevented the growth of a leukemic cell line.[3]
Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds.

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts, and inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B-cell lymphoma-extra large) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress.[1] LIF is capable of maintaining the totipotency of embryonic stem cells.[3] It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.[2]

Physical form

Lyophilized with no additives

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protective effects of leukemia inhibitory factor against oxidative stress during high glucose-induced apoptosis in podocytes.

Xu J et al

Cell Stress & Chaperones, 17(4) (2012)

Leukaemia inhibitory factor is identical to the myeloid growth factor human interleukin for DA cells.

J F Moreau et al.

Nature, 336(6200), 690-692 (1988-12-15)

Leukaemia inhibitory factor (LIF) is a cytokine that induces macrophage differentiation of the murine M1 myeloid leukaemia cell line. We have isolated a cDNA clone encoding a novel human haemopoietic growth factor, human interleukin for DA cells (HILDA) that supports

Reduced immunogenicity of induced pluripotent stem cells derived from Sertoli cells.

Xiaoying Wang et al.

PloS one, 9(8), e106110-e106110 (2014-08-29)

Sertoli cells constitute the structural framework in testis and provide an immune-privileged environment for germ cells. Induced pluripotent stem cells (iPS cells) resemble embryonic stem cells (ES cells) and are generated from somatic cells by expression of specific reprogramming transcription

Critical functions of RhoB in support of glioblastoma tumorigenesis.

Yufang Ma et al.

Neuro-oncology, 17(4), 516-525 (2014-09-14)

RhoB is a member of the Rho small GTPase family that regulates cytoskeletal dynamics and vesicle trafficking. The RhoB homologs, RhoA and RhoC, have been shown to promote cancer progression and metastasis. In contrast, the functions of RhoB in human

Complex conserved organization of the mammalian leukemia inhibitory factor gene: regulated expression of intracellular and extracellular cytokines.

Haines BP et al

Journal of Immunology, 162(8), 4637-4646 (1999)

View All Related Papers

Articles

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

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LIF human