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SRP3318

Sigma-Aldrich

C1 Inhibitor Peptide

≥95% (HPLC), lyophilized powder, human recombinant, expressed in CHO cells

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Synonym(s):
C1inh, Plasma protease C1 inhibitor, Serpin G1
UNSPSC Code:
12352202
NACRES:
NA.32

product name

C1 Inhibitor human, recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

biological source

human

recombinant

expressed in CHO cells

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

potency

≤2.6 nM IC50

mol wt

52.8 kDa

packaging

pkg of 200 μg

impurities

endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SERPING1(710)

General description

Plasma protease C1 inhibitor is a member of the serpin family of structurally related proteins. Recombinant Human C1 inhibitor is a highly glycosylated glycoprotein containing 478 amino acid residues (52.8kDa), corresponding to amino acids 23-500 of the C1 inhibitor precursor, and is fully functional in its ability to inhibit the C1 complex. Glycosylated C1 inhibitor migrates at an apparent molecular weight of approximately 80-90kDa by SDS PAGE analysis under reducing conditions.

Biochem/physiol Actions

Plasma protease C1 inhibitor is the primary regulator of the immune complement system. C1 inhibitor is a protease inhibitor that functions to inhibit the complement system in order to prevent over-activation or spontaneous activation. Inhibition is achieved by binding to and irreversibly inhibiting the C1r and C1s proteases of the C1 complex, which has the effect of shutting down all subsequent downstream events in the complement activation cascade. C1 inhibitor can also inhibit various other proteases, including Kallikrein, Factor XIa, and Factor XIIa. Deficiencies in C1 inhibitor are the primary cause of hereditary angioedema (HAE, hereditary angioneurotic edema), a disease characterized by edema in the respiratory and gastrointestinal tracts. In certain clinical situations, the direct administration of C1 inhibitor can be used to treat HAE and certain other conditions.

Physical form

Lyophilized from 10mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sayani Mukherjee et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(41), 7965-7979 (2020-09-06)
Microglia, a type of CNS immune cell, have been shown to contribute to ethanol-activated neuronal death of the stress regulatory proopiomelanocortin (POMC) neuron-producing β-endorphin peptides in the hypothalamus in a postnatal rat model of fetal alcohol spectrum disorders. We determined
C1-inhibitor deficiency and angioedema.
Carugati A
Molecular Immunology, 38(2-3), 161-173 (2001)
C1 inhibitor.
A E Davis et al.
Methods in enzymology, 223, 97-120 (1993-01-01)
In vivo biosynthesis of endogenous and of human C1 inhibitor in transgenic mice: tissue distribution and colocalization of their expression.
Vinci G
Journal of Immunology, 169(10), 5948-5954 (2002)
C1 Esterase Inhibitor (Human)
P & T : A Peer-Reviewed Journal for Formulary Management, 35, 2-3 (2010)

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